| Flow cytometry immunophenotyping of hematolymphoid neoplasia. | |
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MedLine Citation:
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PMID: 21116989 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Flow cytometry immunophenotyping (FCI) of hematolymphoid specimens is a powerful tool in clinical medicine aiding in the accurate diagnosis and subclassification of acute and chronic leukemias, non-Hodgkin lymphomas, myelodysplasia, and other hematolymphoid neoplastic processes. Multiple flow cytometric strategies are used to evaluate hematolymphoid populations, including identification of neoplastic populations with aberrant immunophenotypes, abnormal maturation patterns, monotypic kappa/lambda light chain expression, restricted V-beta expression, and abnormal light scatter properties. In this chapter, we present a general approach to analyze hematolymphoid populations. Specimen handling, gating strategies, and appropriate antibody panels for flow cytometric detection of abnormal hematologic populations are presented. |
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Authors:
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Katherine R Calvo; Catharine S McCoy; Maryalice Stetler-Stevenson |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 699 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2011 |
Date Detail:
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Created Date: 2010-11-30 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 295-316 Citation Subset: IM |
Affiliation:
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Department of Laboratory Medicine, Hematology Section, National Institutes of Health Clinical Center, Bethesda, MD, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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