| Flow cytometry immunophenotyping of fine-needle aspiration specimens: utility in the diagnosis and classification of non-Hodgkin lymphomas. | |
| | |
MedLine Citation:
|
PMID: 21438908 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Barrena S, Almeida J, García-Macias M D C, López A, Rasillo A, Sayagués J M, Rivas R A, Gutiérrez M L, Ciudad J, Flores T, Balanzategui A, Caballero M D & Orfao A (2011) Histopathology Flow cytometry immunophenotyping of fine-needle aspiration specimens: utility in the diagnosis and classification of non-Hodgkin lymphomas Aims: To establish the utility of flow cytometry (FCM) for screening and diagnosis of B cell non-Hodgkin lymphoma (B-NHL) from lymphoid tissue samples obtained by fine-needle aspiration (FNA). Methods and results: We compared prospectively FCM versus cytology/histology analysis of FNA samples for the diagnostic screening and further World Health Organization (WHO) subclassification of B-NHL. FCM and cytology showed a high degree of agreement (93%); however, diagnosis of reactive processes (RP), B-NHL and T-NHL by FCM showed higher sensitivity than cytology (92-100% versus 64-94%, respectively), without false positive NHL cases. The antibody combination used did not allow a positive diagnosis of Hodgkin lymphoma as distinct from a RP. A high concordance rate was found between FCM and histopathology (74%) in subtyping B-NHL. In this regard, mantle-cell lymphoma and chronic lymphocytic leukaemia/small lymphocytic lymphoma showed the highest degree of agreement (100% concordant rates). In turn, FCM showed higher sensitivity/specificity in classifying follicular lymphoma (FL) and large B cell lymphomas, while the opposite occurred for marginal-zone and lymphoplasmacytic lymphomas. Conclusions: FCM enhances the diagnostic ability of FNA cytology, playing a crucial role in a rapid and accurate differential diagnosis between RP, B-NHL and T-NHL. In addition, immunophenotyping of FNA samples contributes to a more precise subclassification of B-NHL when combined with histopathology and genetic/molecular data. |
| | |
Authors:
|
Susana Barrena; Julia Almeida; María Del Carmen García-Macias; Antonio López; Ana Rasillo; Jose María Sayagués; Rosa Ana Rivas; María Laura Gutiérrez; Juana Ciudad; Teresa Flores; Ana Balanzategui; María Dolores Caballero; Alberto Orfao |
Related Documents
:
|
6966038 - Angiographic diagnosis of acoustic neurinomas and meningiomas in the cerebellopontine a... 19589418 - Mri findings of intracranial cystic meningiomas. 6516918 - Clinical pathology of malignant meningiomas. 20090238 - Meningeal chondroma in a young female. 3399258 - Technique for incisional biopsy of a lacrimal gland mass when the diagnosis of benign m... 8133158 - The use of the rigid endoscope in trans-sphenoidal pituitary surgery. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-3-25 |
Journal Detail:
|
Title: Histopathology Volume: - ISSN: 1365-2559 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
|
Created Date: 2011-3-28 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7704136 Medline TA: Histopathology Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
© 2011 Blackwell Publishing Limited. |
Affiliation:
|
Servicio General de Citometría, Departamento de Medicina and Centro de Investigación del Cáncer (IBMCC-CSIC/USAL), Universidad de Salamanca, Salamanca, Spain Departamento de Anatomía Patológica, Hospital Universitario de Salamanca, Salamanca, Spain Unidad de Biología Molecular, Servicio de Hematología, Hospital Universitario de Salamanca, Salamanca, Spain Servicio de Hematología, Hospital Universitario de Salamanca, Salamanca, Spain. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: MELF invasion in endometrial cancer as a risk factor for lymph node metastasis.
Next Document: Nuclear translocation of ?-catenin and decreased expression of epithelial cadherin in human papillom...