| Flow cytometry enumeration of apoptotic cancer cells by apoptotic rate. | |
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MedLine Citation:
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PMID: 18175809 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Most authors currently quantify the frequency of apoptotic cells in a given phenotypically defined population after calculating the apoptotic index (AI), that is, the percentage of apoptotic cells displaying a specific lineage antigen (LAg) within a population of cells that remain unfragmented and retain the expression of the LAg. However, this approach has two major limitations. First, apoptotic cells fragment into apoptotic bodies that later disintegrate. Second, apoptotic cells frequently lose, partially or even completely, the cell surface expression of the LAg used for the identification of specific cell subsets. This chapter will describe a flow cytometry method to calculate the apoptotic rate (AR) that takes into account both cell fragmentation and loss of LAg expression on measurement of apoptosis using flow cytometry ratiometric cell enumeration that emerges as a more accurate method of measurement of the occurrence of apoptosis in normal and tumoral cell cultures. |
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Authors:
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David Diaz; Alfredo Prieto; Eduardo Reyes; Hugo Barcenilla; Jorge Monserrat; Melchor Alvarez-Mon |
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Publication Detail:
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Type: Journal Article; Review |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 414 ISSN: 1064-3745 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2008 |
Date Detail:
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Created Date: 2008-01-07 Completed Date: 2008-02-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 23-33 Citation Subset: IM |
Affiliation:
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CNB-CSIC R&D Associated Unit, Department of Medicine, University of Alcalá, Alcalá de Henares, Maddrid, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis*
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physiology Cell Count / methods Flow Cytometry / methods* Humans Subcellular Fractions / physiology Tumor Cells, Cultured / cytology* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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