Document Detail

Flow cytometry enumeration of apoptotic cancer cells by apoptotic rate.
MedLine Citation:
PMID:  18175809     Owner:  NLM     Status:  MEDLINE    
Most authors currently quantify the frequency of apoptotic cells in a given phenotypically defined population after calculating the apoptotic index (AI), that is, the percentage of apoptotic cells displaying a specific lineage antigen (LAg) within a population of cells that remain unfragmented and retain the expression of the LAg. However, this approach has two major limitations. First, apoptotic cells fragment into apoptotic bodies that later disintegrate. Second, apoptotic cells frequently lose, partially or even completely, the cell surface expression of the LAg used for the identification of specific cell subsets. This chapter will describe a flow cytometry method to calculate the apoptotic rate (AR) that takes into account both cell fragmentation and loss of LAg expression on measurement of apoptosis using flow cytometry ratiometric cell enumeration that emerges as a more accurate method of measurement of the occurrence of apoptosis in normal and tumoral cell cultures.
David Diaz; Alfredo Prieto; Eduardo Reyes; Hugo Barcenilla; Jorge Monserrat; Melchor Alvarez-Mon
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  414     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2008  
Date Detail:
Created Date:  2008-01-07     Completed Date:  2008-02-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  23-33     Citation Subset:  IM    
CNB-CSIC R&D Associated Unit, Department of Medicine, University of Alcalá, Alcalá de Henares, Maddrid, Spain.
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MeSH Terms
Apoptosis* / physiology
Cell Count / methods
Flow Cytometry / methods*
Subcellular Fractions / physiology
Tumor Cells, Cultured / cytology*

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