| Flow cytometry for the assessment of animal sperm integrity and functionality: state of the art. | |
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MedLine Citation:
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PMID: 21478895 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Flow cytometry is now a recognized methodology within animal spermatology, and has moved from being a research tool to become routine in the assessment of animal semen destined to breeding. The availability of 'bench-top' flow cytometers and of newer and versatile markers for cell structure and function had allowed the instrumentation to measure more sperm parameters, from viability to reactiveness when exposed to exogenous stimuli, and to increase our capabilities to sort spermatozoa for potential fertilizing capacity, or chromosomal sex. The present review summarizes the state of the art regarding flow cytometry applied to animal andrology, albeit keeping an open comparative intent. It critically evaluates the present and future capabilities of flow cytometry for the diagnostics of potential fertility and for the development of current reproductive technologies such as sperm freezing, sperm selection and sperm sorting. The flow cytometry methods will probably further revolutionize our understanding of the sperm physiology and their functionality, and will undoubtedly extend its application in isolating many uncharacterized features of spermatozoa. However, continuous follow-up of the methods is a necessity owing to technical developments and the complexity of mapping spermatozoa.Asian Journal of Andrology advance online publication, 11 April 2011; doi:10.1038/aja.2011.15. |
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Authors:
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Md Sharoare Hossain; Anders Johannisson; Margareta Wallgren; Szabolcs Nagy; Amanda Pimenta Siqueira; Heriberto Rodriguez-Martinez |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-4-11 |
Journal Detail:
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Title: Asian journal of andrology Volume: - ISSN: 1745-7262 ISO Abbreviation: - Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-4-11 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100942132 Medline TA: Asian J Androl Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1] Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences (SLU), SE-750 07 Uppsala, Sweden [2] Division of Reproduction, SLU, SE-750 07 Uppsala, Sweden. |
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