| Flow cytometry as a tool in the evaluation of blood leukocyte function in Chelonia mydas (Linnaeus, 1758) (Testudines, Cheloniidae). | |
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MedLine Citation:
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PMID: 19802451 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Chelonia mydas is a sea turtle that feeds and nests on the Brazilian coast and a disease called fibropapillomatosis is a threat to this species. Because of this, it is extremely necessary to determine a methodology that would enable the analysis of blood leukocyte function in these sea turtles. In order to achieve this aim, blood samples were collected from C. mydas with or without fibropapillomas captured on the São Paulo north coast. Blood samples were placed in tubes containing sodium heparin and were transported under refrigeration to the laboratory in sterile RPMI 1640 cell culture medium. Leukocytes were separated by density gradient using Ficoll-PaqueTM Plus, Amershan Biociences. The following stimuli were applied in the assessment of leukocyte function: Phorbol Miristate-Acetate (PMA) for oxidative burst activity evaluation and Zymosan A (Saccharomyces cerevisiae) Bio Particles, Alexa Fluor 594 conjugate for phagocytosis evaluation. Three cell populations were identified: heterophils, monocytes and lymphocytes. Monocytes were the cells responsible for phagocytosis and oxidative burst. |
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Authors:
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S Rossi; V M Sá-Rocha; D Kinoshita; A Genoy-Puerto; T Zwarg; M R Werneck; L C Sá-Rocha; E R Matushima |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Brazilian journal of biology = Revista brasleira de biologia Volume: 69 ISSN: 1678-4375 ISO Abbreviation: Braz J Biol Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-10-05 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101129542 Medline TA: Braz J Biol Country: Brazil |
Other Details:
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Languages: eng Pagination: 899-905 Citation Subset: IM |
Affiliation:
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Programa de Pós-Graduação em Patologia Experimental e Comparada, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brazil 05508-900. rossi.silmara@yahoo.com.br |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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