| Flow cytometry as a diagnostic tool for hereditary spherocytosis. | |
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MedLine Citation:
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PMID: 17016037 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Flow cytometric analysis of eosin-5'-maleimide-labeled red blood cells has been proposed as a new method of identifying hereditary spherocytosis (HS). The aim of the present study was to analyze sensitivity and specificity of this method. Red blood cells from patients with HS (n = 58) revealed significantly lower mean channel fluorescence values than red blood cells from normal subjects (n = 110), unaffected HS family members (n = 8), and patients with other anemias (n = 44). Taking a mean channel fluorescence of 400.0 units as the threshold value identified by logistic regression, sensitivity and specificity of the test for HS were 96.6 and 99.1%, respectively. Flow cytometric analysis is a valuable screening test for the diagnosis of HS. |
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Authors:
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Gudrun Stoya; Bernd Gruhn; Heinz Vogelsang; Eckehard Baumann; Werner Linss |
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Publication Detail:
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Type: Evaluation Studies; Journal Article |
Journal Detail:
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Title: Acta haematologica Volume: 116 ISSN: 0001-5792 ISO Abbreviation: Acta Haematol. Publication Date: 2006 |
Date Detail:
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Created Date: 2006-10-03 Completed Date: 2006-10-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0141053 Medline TA: Acta Haematol Country: Switzerland |
Other Details:
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Languages: eng Pagination: 186-91 Citation Subset: IM |
Copyright Information:
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Copyright 2006 S. Karger AG, Basel. |
Affiliation:
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Institute of Anatomy I, Friedrich Schiller University, Jena, Germany. gsto@mti.uni-jena.de |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anemia, Hemolytic
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diagnosis Eosine Yellowish-(YS) / analogs & derivatives*, diagnostic use Erythrocytes / chemistry* Flow Cytometry / methods* Humans Logistic Models Sensitivity and Specificity Spherocytosis, Hereditary / diagnosis* |
| Chemical | |
Reg. No./Substance:
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17372-87-1/Eosine Yellowish-(YS); 76296-42-9/eosin maleimide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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