Document Detail

Flow cytometry analysis of cell cycle in myosin II-deficient yeast.
MedLine Citation:
PMID:  10028539     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To determine whether cell cycle changes can be detected in myosin II-deficient cells using flow cytometry techniques.
BACKGROUND: Although the primary role of myosin II (Myo1p) in the yeast Saccharomyces cerevisiae is in cytokinesis we have reported that this conventional myosin also appears to influence the regulation of cell wall metabolism as indicated by increases in the expression of chitin metabolizing enzymes in a null mutant of the MYO1 gene. The expression of these enzymes is known to be regulated in the cell cycle suggesting that cell cycle changes may alter their expression.
METHODS: Flow cytometry was employed to assess the nuclear DNA content of logarithmic yeast cell cultures as a means of determining changes in the cell cycle of Myo1p-deficient cells.
RESULTS: Significant changes were observed in the Myo1p-deficient strain suggesting that these cells are arrested in G2/M-phase of the cell cycle.
CONCLUSIONS: Based on the results of this preliminary study, we propose a model in which the increased activity of chitin metabolizing enzymes may be explained by a mitotic arrest in these cells.
J A Cruz; L Villanueva; J R Rodríguez-Medina
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Puerto Rico health sciences journal     Volume:  17     ISSN:  0738-0658     ISO Abbreviation:  P R Health Sci J     Publication Date:  1998 Dec 
Date Detail:
Created Date:  1999-04-19     Completed Date:  1999-04-19     Revised Date:  2013-04-26    
Medline Journal Info:
Nlm Unique ID:  8303541     Medline TA:  P R Health Sci J     Country:  PUERTO RICO    
Other Details:
Languages:  eng     Pagination:  323-6     Citation Subset:  IM    
Department of Biochemistry, University of Puerto Rico, School of Medicine, San Juan 365067, USA.
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MeSH Terms
Cell Culture Techniques
Cell Cycle
Cell Division
Cell Wall / metabolism
Chitin / metabolism
Chitin Synthase / genetics,  metabolism
Flow Cytometry
Gene Expression
Myosin Heavy Chains / deficiency,  genetics,  metabolism*
RNA, Messenger / genetics
Saccharomyces cerevisiae / cytology,  genetics,  metabolism
Yeasts / cytology*,  genetics,  metabolism*
Grant Support
Reg. No./Substance:
0/Myosin Heavy Chains; 0/RNA, Messenger; 1398-61-4/Chitin; EC Synthase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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