Document Detail

Flow cytometrical and genotypic analysis of primary non-Hodgkin's lymphoma of bone.
MedLine Citation:
PMID:  9406249     Owner:  NLM     Status:  MEDLINE    
Primary malignant lymphoma of bone presents both diagnostic and therapeutic problems. No previous study has addressed the use of flow cytometry in the immunophenotypic evaluations of bone lymphomas. A 41-year-old Japanese female presented with a large lytic lesion in the right femur. Biopsies from the lesion were examined by light microscopy, immunohistochemistry, 3-color flow cytometry and Southern blotting. Microscopic examination showed a diffuse, noncleaved large cell lymphoma. The lymphoid cells were positive for mature B cell antigens and the phenotype was: CD5-, CD10-, CD19+, 20+, CD22+, IgG+, IgA-, IgM-, kappa-, lambda+, CD1-, CD2-, CD3-, CD4-, CD7-, CD8-. Southern blot analysis revealed rearranged bands on both heavy- and lambda light-chain genes, in contrast to germline configuration on T cell antigen receptor (beta, gamma and delta) genes. Flow cytometry, in conjunction with morphologic and other molecular techniques, can provide a rapid and accurate means of diagnosing primary lymphoma of bone. In addition, the capabilities of flow cytometry to assess cell properties/constituents can be utilized in detailed analysis of adhesion molecules and activation antigen which may lead to better prediction of the prognosis of this group of lymphomas, and may provide further important data for the therapeutic decision making process.
M Hatori; R Ichinohasama; J Myers; A Schiller; S Kokubun
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Publication Detail:
Type:  Case Reports; Journal Article    
Journal Detail:
Title:  Pathology, research and practice     Volume:  193     ISSN:  0344-0338     ISO Abbreviation:  Pathol. Res. Pract.     Publication Date:  1997  
Date Detail:
Created Date:  1998-01-16     Completed Date:  1998-01-16     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  7806109     Medline TA:  Pathol Res Pract     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  557-64     Citation Subset:  IM    
Department of Orthopaedic Surgery, Tohoku University School of Medicine, Sendai, Japan.
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MeSH Terms
Blotting, Southern
Bone Neoplasms / chemistry,  genetics,  pathology*
Flow Cytometry*
Immunoglobulin Heavy Chains / genetics
Immunoglobulin Light Chains / genetics
Lymphoma, Non-Hodgkin / chemistry,  genetics*,  pathology*
Reg. No./Substance:
0/Immunoglobulin Heavy Chains; 0/Immunoglobulin Light Chains

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