| Flow cytometric studies of nucleoside transport regulation in single chromaffin cells. | |
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MedLine Citation:
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PMID: 9498818 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The present paper reveals that a fluorescent derivative of nitrobenzylthioinosine, 5-(SAENTA-x8)-fluorescein, is a highly specific inhibitor of the neural NBTI-sensitive nucleoside transporter. 5-(SAENTA-x8)-fluorescein inhibited adenosine transport and [3H]NBTI binding with a Ki of 4 nM in cultured chromaffin cells. Flow cytometry demonstrated that 5-(SAENTA-x8)-fluorescein specifically interacted with the NBTI-sensitive nucleoside transporters with high affinity (K[D] = 6 nM). Activation of protein kinases A and C with forskolin or nicotinic receptor agonists, respectively, resulted in 50% inhibition of the fluorescence bound to the cells. Flow cytometry will allow studying nucleoside transport in single cells from heterogeneous neural cell populations. |
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Authors:
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R P Sen; E G Delicado; A Alvarez; A M Brocklebank; J S Wiley; M T Miras-Portugal |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEBS letters Volume: 422 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1998 Feb |
Date Detail:
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Created Date: 1998-03-19 Completed Date: 1998-03-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 368-72 Citation Subset: IM |
Affiliation:
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Departamento de Bioquímica, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine
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metabolism Animals Carrier Proteins / metabolism* Cattle Cells, Cultured Chromaffin Cells / metabolism* Flow Cytometry Fluoresceins / metabolism* Membrane Proteins / metabolism* Nucleoside Transport Proteins Purine Nucleosides / metabolism* Thioinosine / analogs & derivatives, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Fluoresceins; 0/Membrane Proteins; 0/Nucleoside Transport Proteins; 0/Purine Nucleosides; 147395-08-2/5-(SAENTA-x8)fluorescein; 38048-32-7/4-nitrobenzylthioinosine; 574-25-4/Thioinosine; 58-61-7/Adenosine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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