Document Detail


Flow cytometric studies of nucleoside transport regulation in single chromaffin cells.
MedLine Citation:
PMID:  9498818     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present paper reveals that a fluorescent derivative of nitrobenzylthioinosine, 5-(SAENTA-x8)-fluorescein, is a highly specific inhibitor of the neural NBTI-sensitive nucleoside transporter. 5-(SAENTA-x8)-fluorescein inhibited adenosine transport and [3H]NBTI binding with a Ki of 4 nM in cultured chromaffin cells. Flow cytometry demonstrated that 5-(SAENTA-x8)-fluorescein specifically interacted with the NBTI-sensitive nucleoside transporters with high affinity (K[D] = 6 nM). Activation of protein kinases A and C with forskolin or nicotinic receptor agonists, respectively, resulted in 50% inhibition of the fluorescence bound to the cells. Flow cytometry will allow studying nucleoside transport in single cells from heterogeneous neural cell populations.
Authors:
R P Sen; E G Delicado; A Alvarez; A M Brocklebank; J S Wiley; M T Miras-Portugal
Related Documents :
19692428 - Differential usage of the transport systems for folic acid and methotrexate in normal h...
8074188 - Hypertonic upregulation of amino acid transport system a in vascular smooth muscle cells.
2825778 - Properties of a na+-coupled serine-threonine transport system in escherichia coli.
12115918 - Transfection of an inducible trout anion exchanger (ae1) into hek-ecr cells.
3613528 - The differentiation capacity of clara cells isolated from the lungs of rabbits.
1588588 - An ultrastructural study of differentiation of pyriform cells and their contribution to...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  422     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1998 Feb 
Date Detail:
Created Date:  1998-03-19     Completed Date:  1998-03-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  368-72     Citation Subset:  IM    
Affiliation:
Departamento de Bioquímica, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adenosine / metabolism
Animals
Carrier Proteins / metabolism*
Cattle
Cells, Cultured
Chromaffin Cells / metabolism*
Flow Cytometry
Fluoresceins / metabolism*
Membrane Proteins / metabolism*
Nucleoside Transport Proteins
Purine Nucleosides / metabolism*
Thioinosine / analogs & derivatives,  metabolism
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Fluoresceins; 0/Membrane Proteins; 0/Nucleoside Transport Proteins; 0/Purine Nucleosides; 147395-08-2/5-(SAENTA-x8)fluorescein; 38048-32-7/4-nitrobenzylthioinosine; 574-25-4/Thioinosine; 58-61-7/Adenosine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Inhibition of HIV-1 Nef-induced apoptosis of uninfected human blood cells by serine/threonine protei...
Next Document:  Isotope dilution GC/MS determination of anandamide and other fatty acylethanolamides in rat blood pl...