Document Detail


Flow cytometric sorting of human sperm: MicroSort clinical trial update.
MedLine Citation:
PMID:  18954899     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This report provides a summary of MicroSort efficacy in separation of X- from Y-chromosome bearing human sperm (XSort and YSort, respectively), clinical outcomes, and the sex of the resultant babies when sorted sperm were used for intrauterine insemination (IUI), in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI). Clinical trial participants were married couples seeking reduced X-linked genetic disorder risk or family balancing. Sperm were stained with Hoechst 33342, sorted by flow cytometry, then used or cryopreserved for subsequent use. Fluorescence in situ hybridization (FISH) analysis determined the post-sort enrichment (purity) for X- and Y-bearing sperm. Birth and pediatric records were evaluated for incidence of congenital malformations. Between June 1994 and January 2007, patients underwent 3629 IUI cycles, 1642 IVF/ICSI cycles with fresh embryo transfer (ET) and 99 frozen embryo transfer (FET) cycles after MicroSort. Of 5871 total sorts, 74.9% were XSort and 25.1% were YSort. IVF/ICSI fertilization rate was 70.7% and 93.8% of 2PN embryos cleaved. The pregnancy rates for IUI, IVF/ICSI, and FET were 15.6, 32.0, and 33.3%, respectively, while miscarriage rates were 15.7, 14.3, and 33.3%, respectively. Post-sort purity averaged 87.9% (XSort and 73.4% (YSort. A total of 1125 clinical pregnancies yielded 943 babies born and 167 ongoing pregnancies. For babies born, XSort resulted in 92.0% females and YSort yielded 81.5% males. Postnatal follow-up showed a 2.6% major congenital malformation rate, with no recurrent pattern or clustering of malformations. FISH results confirmed MicroSort enrichment of X- and Y-bearing sperm populations that closely corresponded with the sex of the resultant child. Fertilization, cleavage, spontaneous abortion, and pregnancy rates as well as incidence of major congenital malformations were comparable to those in literature reports utilizing unsorted sperm.
Authors:
D S Karabinus
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Publication Detail:
Type:  Clinical Trial; Journal Article     Date:  2008-10-26
Journal Detail:
Title:  Theriogenology     Volume:  71     ISSN:  0093-691X     ISO Abbreviation:  Theriogenology     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-12-01     Completed Date:  2009-03-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  74-9     Citation Subset:  IM    
Affiliation:
MicroSort Division, Genetics & IVF Institute, Fairfax, VA 22031, USA. dkarabinus@givf.com
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MeSH Terms
Descriptor/Qualifier:
Adult
Chromosomes, Human, X
Chromosomes, Human, Y
Cryopreservation
Female
Flow Cytometry / methods*
Humans
Male
Pregnancy
Semen Preservation / methods
Sex Determination (Analysis) / methods*
Spermatozoa / physiology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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