| Flow cytometric methods for prenatal and neonatal diagnosis. | |
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MedLine Citation:
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PMID: 20932840 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Flow cytometry offers a promising alternative to the current methods of amniocentesis or chorionic villus sampling (CVS) for fetal cell sorting for prenatal diagnosis. While flow cytometric methods have been greatly improved to be more sensitive at detecting fetal cells within the maternal circulation, there are still several challenges that need to be overcome before application in prenatal diagnosis. However, flow cytometry is a powerful tool that can be used to enhance molecular testing and other diagnostic testing modalities in prenatal and neonatal diagnosis. It remains the gold standard to identify cellular immunodeficiencies and, for some immunological disorders with established biomarkers, flow cytometric assays can be used to make a definitive diagnosis. In this review, the advantages and disadvantages of using MACS and FACS analysis for fetal cell sorting are discussed. This review also includes an overview of the current flow cytometric assays and biomarkers that may be used for prenatal and neonatal diagnosis of common immunological and hematological abnormalities and the role of flow cytometry in treatment monitoring after bone marrow and stem cell transplantation. |
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Authors:
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Michèle G Curtis; Brooke Walker; Thomas N Denny |
Publication Detail:
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Type: Journal Article; Review Date: 2010-10-13 |
Journal Detail:
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Title: Journal of immunological methods Volume: 363 ISSN: 1872-7905 ISO Abbreviation: J. Immunol. Methods Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-12-17 Completed Date: 2011-01-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1305440 Medline TA: J Immunol Methods Country: Netherlands |
Other Details:
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Languages: eng Pagination: 198-209 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Obstetrics and Gynecology, University of Texas, Houston, TX 77026, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biological Markers
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blood Cell Separation / methods Female Fetal Diseases / blood, diagnosis*, immunology Flow Cytometry / methods* Humans Infant, Newborn Pregnancy Prenatal Diagnosis / methods* |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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