| Flow cytometric method using fluorescent microspheres to measure reticuloendothelial function or particulate translocation. | |
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MedLine Citation:
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PMID: 10711506 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The reticuloendothelial system (RES) influences the outcome of vascular shock and environmental stress. We describe a procedure that employs flow cytometry and 1 microm fluorescent microspheres (FM) to study RES function. FM (2 x 10(10) beads/kg) were administered via a jugular cannula in Sprague-Dawley rats. After 15 min, blood and tissues were collected and digested in 15% KOH. Phycoerythrin 1 microm beads were added to each sample as an internal standard and analyzed by flow cytometry. FM were preferentially cleared by the spleen, liver and lung. Clearance was confirmed by fluorescent photomicroscopy. Addition of the internal standard to determine accurately aspiration volume enhanced precision. This procedure offers advantages over other RES clearance methods including bacterial, radioactive or carbon clearance assays. Moreover, this method could enhance accuracy, reproducibility and speed of data collection in particulate transport studies that are based on manual microscopic scanning and FM counting. |
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Authors:
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D A DuBose; C Matthew; E Schroder; C Repique; J Balcius; D Morehouse; I Sils |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biotechnic & histochemistry : official publication of the Biological Stain Commission Volume: 74 ISSN: 1052-0295 ISO Abbreviation: Biotech Histochem Publication Date: 1999 Sep |
Date Detail:
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Created Date: 2000-04-28 Completed Date: 2000-04-28 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9107378 Medline TA: Biotech Histochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 261-5 Citation Subset: IM |
Affiliation:
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U.S. Army Research Institute of Environmental Medicine, Natick, Massachusetts 01760, USA. ddubose@natick-ccmail.army.mil |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Flow Cytometry / methods* Fluorescein* Fluorescent Dyes* Male Microspheres Mononuclear Phagocyte System / pathology, physiology* Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 2321-07-5/Fluorescein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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