| Flow-cytometric investigation of epidermal cell characteristics in monogenic disorders of keratinization and their modulation by topical calcipotriol treatment. | |
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MedLine Citation:
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PMID: 8740259 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A flow-cytometric study was performed in monogenic disorders of keratinization, to assess DNA distribution as well as the expression of keratins and involucrin. In addition, the changes in expression of these markers under influence of calcipotriol treatment were investigated. Proliferation, measured by the percentage of epidermal cells in SG2M-phase of the cell cycle, was increased in Darier's disease, lamellar ichthyosis, congenital bullous ichthyotic erythroderma of Brocq and the Comel-Netherton syndrome, whereas normal proliferation was found in autosomal dominant ichthyosis vulgaris, X-linked recessive ichthyosis, keratosis pilaris, ichthyosis bullosa of Siemens and the Sjögren-Larsson syndrome. Keratin 6 was enhanced in erythrodermic lamellar ichthyosis, congenital bullous ichthyotic erythroderma of Brocq and the Comel-Netherton syndrome, showing also reduction of keratin 10. Involucrin was only slightly reduced in erythrodermic lamellar ichthyosis, congenital bullous ichthyotic erythroderma of Brocq and the Comel-Netherton syndrome, compared to the pronounced reduction in all other skin disorders studied. Calcipotriol was found to enhance differentiation in Darier's disease, erythrodermic lamellar ichthyosis, and congenital bullous ichthyotic erythroderma of Brocq. Only Darier's disease did not show clinical improvement. In conclusion, flow cytometry provides a useful method for quantification of epidermal cell characteristics in monogenic disorders of keratinization. Further studies need to be performed to establish its usefulness as a diagnostic and prognostic tool. |
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Authors:
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G P Lucker; P M Steijen; E J Suykerbuyk; K Kragballe; F Brandrup; P C van de Kerkhof |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Acta dermato-venereologica Volume: 76 ISSN: 0001-5555 ISO Abbreviation: Acta Derm. Venereol. Publication Date: 1996 Mar |
Date Detail:
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Created Date: 1996-11-18 Completed Date: 1996-11-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370310 Medline TA: Acta Derm Venereol Country: NORWAY |
Other Details:
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Languages: eng Pagination: 97-101 Citation Subset: IM |
Affiliation:
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Department of Dermatology, University Hospital, Nijmegen, The Netherlands. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Administration, Topical Calcitriol / administration & dosage, analogs & derivatives*, therapeutic use Cell Division / drug effects Cells, Cultured DNA / analysis* Dermatologic Agents / administration & dosage, therapeutic use* Epidermis / cytology* Flow Cytometry Humans Ichthyosis / drug therapy, genetics, pathology Keratosis / drug therapy*, genetics, pathology* Reference Values Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Dermatologic Agents; 112965-21-6/calcipotriene; 32222-06-3/Calcitriol; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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