| Flow cytometric immunophenotypic assessment of T-cell clonality by vβ repertoire analysis in fine-needle aspirates and cerebrospinal fluid. | |
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MedLine Citation:
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PMID: 22261447 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Flow cytometric T-cell receptor V(β) repertoire analysis (TCR-V(β)-R) is a sensitive method to detect T-cell clonality; however, its implementation in low-cellularity specimens has not been established. We developed a strategy to use TCR-V(β)-R in cerebrospinal fluid (CSF) and fine-needle aspirate (FNA) specimens. Initially, full TCR-V(β)-R was evaluated in diagnostic/screening specimens from 8 patients with T-cell neoplasia to determine tumor-specific TCR-V(β) protein expression. Subsequently, an abbreviated, patient-specific TCR-V(β)-R evaluation was performed in 17 paucicellular specimens from the patients (8 CSF, 9 FNA) for staging and monitoring of minimal residual disease (MRD). A single cocktail containing 3 anti-V(β) antibodies (1 tumor-specific and 2 negative controls) in combination with other antibodies chosen to help gate on atypical T cells is highly sensitive and specific for detecting low-level neoplastic T-cell involvement in paucicellular specimens. This TCR-V(β)-R strategy is valuable in staging and evaluating MRD in patients with T-cell non-Hodgkin lymphoma. |
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Authors:
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Prashant Tembhare; Constance M Yuan; John C Morris; John E Janik; Armando C Filie; Maryalice Stetler-Stevenson |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: American journal of clinical pathology Volume: 137 ISSN: 1943-7722 ISO Abbreviation: Am. J. Clin. Pathol. Publication Date: 2012 Feb |
Date Detail:
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Created Date: 2012-01-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370470 Medline TA: Am J Clin Pathol Country: United States |
Other Details:
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Languages: eng Pagination: 220-6 Citation Subset: AIM; IM |
Affiliation:
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Flow Cytometry Unit, Laboratory of Pathology, NCI, NIH, Bldg 10, Room 2A-33, Mail Stop 1500, Bethesda, MD, 20892. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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