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Flow cytometric identification of Mamiellales clade II in the Southern Atlantic Ocean.
MedLine Citation:
PMID:  23035812     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Flow cytometric sorting, based on cellular optical properties and macromolecule content, has been successfully employed to taxonomically affiliate bacterioplankton. However, this approach has not been much used for eukaryotic plankton. To redress this imbalance we identified a conspicuous group of red autofluorescent picoplankton in surface waters of the South Atlantic Ocean. Using catalysed reporter deposition fluorescence in situ hybridization virtually all cells sorted from that group were affiliated with the Mamiellales clade II (84 ± 4%, division Chlorophyta) with a size of 1.6 ± 0.03 μm. Based on electron microscopy the Mamiellales clade II sorted cells have a simple morphology with apparently no scales, flagella, or surface features. Their latitudinal distribution resembled the distribution of Synechococcus with very low concentrations in the surface waters of the Southern subtropical gyre (0.6 - 1.6 × 10(3) cells ml(-1) ) and increased concentrations in the Southern temperate waters 8.3 × 10(3) cells ml(-1) . Identification of the flow cytometric group as Mamiellales clade II allowed us to characterise the morphology of these enigmatic uncultured picoplanktonic cells by electron microscopy and to determine their apparent preference for temperate rather than subtropical oceanic photic waters. © 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Authors:
Paola R Gómez-Pereira; Gabrielle Kennaway; Bernhard M Fuchs; Glen A Tarran; Mikhail V Zubkov
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-5
Journal Detail:
Title:  FEMS microbiology ecology     Volume:  -     ISSN:  1574-6941     ISO Abbreviation:  FEMS Microbiol. Ecol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8901229     Medline TA:  FEMS Microbiol Ecol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Affiliation:
National Oceanography Centre, Ocean Biogeochemistry & Ecosystems Research Group, European Way, Southampton, SO14 3ZH, United Kingdom.
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