Document Detail


Flow cytometric evaluation of epithelial ovarian cancer.
MedLine Citation:
PMID:  2360592     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the prognostic significance of deoxyribonucleic acid content and proliferative activity of tumor cell populations as measured by flow cytometry of the tumor specimens from 115 women with epithelial ovarian cancer. Deoxyribonucleic acid aneuploidy was found in 87 of 115 (76%) of these cancers with a mean deoxyribonucleic acid index of 1.6 and S-phase fraction of 14.7%. The S-phase fraction of the 28 (24%) diploid tumors was 7.0%. Deoxyribonucleic acid ploidy was significantly correlated with survival. S-phase fraction was significantly correlated with ploidy, residual tumor, histology, grade, ascites, time to recurrence, and survival. Diploidy versus aneuploidy were the best discriminating values for deoxyribonucleic acid index and an S-phase fraction of greater or less than 18% for that parameter. Multivariate analysis revealed stage, S-phase fraction, residual tumor, and grade to be independently associated with time to recurrence, and stage, age, S-phase fraction, and largest metastases were factors associated with survival. Deoxyribonucleic acid ploidy did not significantly improve either model. These results suggest that abnormalities of deoxyribonucleic acid content and the proliferative activity of tumor cell populations are reflective of their biologic activity.
Authors:
V M Barnabei; D S Miller; K D Bauer; T M Murad; A W Rademaker; J R Lurain
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of obstetrics and gynecology     Volume:  162     ISSN:  0002-9378     ISO Abbreviation:  Am. J. Obstet. Gynecol.     Publication Date:  1990 Jun 
Date Detail:
Created Date:  1990-08-02     Completed Date:  1990-08-02     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370476     Medline TA:  Am J Obstet Gynecol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1584-90; discussion 1590-2     Citation Subset:  AIM; IM    
Affiliation:
Department of Obstetrics and Gynecology, Northwestern University Medical School, Chicago, IL.
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MeSH Terms
Descriptor/Qualifier:
Aneuploidy*
DNA, Neoplasm / analysis
Diploidy
Epithelium / analysis,  pathology
Female
Flow Cytometry*
Humans
Neoplasm Staging
Ovarian Neoplasms / analysis*,  diagnosis,  pathology
Prognosis
Recurrence
Grant Support
ID/Acronym/Agency:
RR-05370/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Neoplasm

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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