Document Detail

Flow cytometric evaluation of cell cycle characteristics during in vitro differentiation of chick embryo chondrocytes.
MedLine Citation:
PMID:  3402279     Owner:  NLM     Status:  MEDLINE    
The cell cycle kinetic characteristics of chick endochondral chondrocytes differentiating in vitro were studied by flow cytometry. In addition, the synthesis of type I and type X collagens of the same cells was evaluated by immunoprecipitation. Dedifferentiated cells, derived from chick embryo tibiae and grown attached to a substratum, were characterized by type I collagen synthesis, a high growth fraction (GF = 0.94), minimal cell loss factor (phi = 0.02), and a total cell cycle time of the proliferating cells of about 17 h (tG1 = 8 h, tS = 5 h, and tG2 + M = 4 h). Transfer of dedifferentiated cells to suspension culture on agarose-coated dishes induced differentiation to hypertrophic chondrocytes. These were characterized by type X collagen synthesis, a low growth fraction (GF = 0.52), maximal cell loss factor (phi = 1.0), and a total cell cycle time of the proliferating cells of about 73 h (tG1 = 53 h, tS = 12 h, and tG2 + M = 8 h). The transition from dedifferentiated chondrocytes to hypertrophic chondrocytes was accompanied by large increases of the duration of all the cell cycle phases and of the number of quiescent and degenerating cells. Associated with these alterations in cell cycle kinetics was a switch from type I to type X collagen synthesis. Further preliminary data suggest that the population of differentiating chondrocytes (a state between dedifferentiated and hypertrophic chondrocytes) comprises a heterogeneous population of fast and slow growing cells.
W Giaretti; G Moro; R Quarto; S Bruno; A Di Vinci; E Geido; R Cancedda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cytometry     Volume:  9     ISSN:  0196-4763     ISO Abbreviation:  Cytometry     Publication Date:  1988 Jul 
Date Detail:
Created Date:  1988-09-13     Completed Date:  1988-09-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8102328     Medline TA:  Cytometry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  281-90     Citation Subset:  IM    
Lab. Biofisica, Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy.
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MeSH Terms
Bromodeoxyuridine / metabolism
Cartilage / cytology*,  metabolism
Cell Cycle
Cell Differentiation
Cell Separation
Cells, Cultured
Chick Embryo
Collagen / biosynthesis
Flow Cytometry*
Time Factors
Reg. No./Substance:
59-14-3/Bromodeoxyuridine; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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