Document Detail


Flow cytometric double labeling technique for screening of multidrug resistance.
MedLine Citation:
PMID:  1685985     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the capabilities of flow cytometry in the analysis of a multidrug resistant (MDR) human ovarian cancer cell line 2780AD and its drug sensitive parental A2780. A functional assay using daunorubicin (DNR) as a fluorescent probe was combined with an immunofluorescence assay of P-glycoprotein (P-gp) using the monoclonal antibody MRK-16. Functionally MDR could be demonstrated by the lower DNR-content of MDR cells compared to DNR-content of drug sensitive cells. When incubation was performed with DNR in the presence of verapamil, DNR-content increased in the MDR cells. However the content of the A2780 cells was never attained. Differences in DNR-content were not related to differences in DNA-content. In experimental cell lines immunofluorescence data were inversely related with those of DNR-content: MDR cells had high levels of P-gp expression and low levels of DNR-content (and vice versa in drug sensitive cells). Both assays can be easily combined in a multiparametric flow cytometric procedure to evaluate both parameters simultaneously in the same cells. Analysis of clinical samples demonstrates the existence of aberrant subpopulations which would not be detected by using a single parameter assay.
Authors:
E E Gheuens; D R van Bockstaele; M van der Keur; H J Tanke; A T van Oosterom; E A De Bruijn
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cytometry     Volume:  12     ISSN:  0196-4763     ISO Abbreviation:  Cytometry     Publication Date:  1991  
Date Detail:
Created Date:  1992-03-16     Completed Date:  1992-03-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8102328     Medline TA:  Cytometry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  636-44     Citation Subset:  IM    
Affiliation:
Lab of Cancer Research and Clinical Oncology, Antwerp University, Wilrijk, Belgium.
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MeSH Terms
Descriptor/Qualifier:
Antibiotics, Antineoplastic / analysis*
Antibodies, Monoclonal
Cell Line, Transformed
Daunorubicin
Drug Resistance*
Female
Flow Cytometry / methods*
Fluorescent Dyes
Humans
Membrane Glycoproteins / analysis*,  metabolism
Ovarian Neoplasms / metabolism
P-Glycoprotein
Chemical
Reg. No./Substance:
0/Antibiotics, Antineoplastic; 0/Antibodies, Monoclonal; 0/Fluorescent Dyes; 0/Membrane Glycoproteins; 0/P-Glycoprotein; 20830-81-3/Daunorubicin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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