| Flow cytometric differentiation of avian leukocytes and analysis of their intracellular cytokine expression. | |
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MedLine Citation:
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PMID: 20390535 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A flow cytometric method for the identification of chicken blood leukocyte subpopulations and thrombocytes was developed. An anti-chicken CD45 phycoerythrin-labelled antibody was used to separate leukocytes from red blood cell nuclei. Leukocytes and thrombocytes were identified using a combination of their CD45-positivity and their typical side scatter properties. The identity of the CD45-positive cells was confirmed by sorting the subpopulations and subsequent light microscopic evaluation. In these differentiated cell populations, intracellular expression analysis of the proinflammatory cytokines interleukin-1beta and interleukin-6 was subsequently optimized on whole blood after in vitro stimulation with lipopolysaccharide from Escherichia coli strain O127:B8. |
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Authors:
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S De Boever; S Croubels; K Demeyere; B Lambrecht; P De Backer; E Meyer |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Avian pathology : journal of the W.V.P.A Volume: 39 ISSN: 1465-3338 ISO Abbreviation: Avian Pathol. Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-04-14 Completed Date: 2010-07-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8210638 Medline TA: Avian Pathol Country: England |
Other Details:
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Languages: eng Pagination: 41-6 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, Toxicology and Biochemistry, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. sandra.deboever@ugent.be |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal Antigens, CD45 / immunology, metabolism* Blood Platelets / cytology, metabolism Cell Separation Chickens Erythrocytes / metabolism Flow Cytometry / methods* Interleukin-1beta / metabolism* Interleukin-6 / metabolism* Leukocytes* / cytology, metabolism Phycoerythrin Staining and Labeling |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Interleukin-1beta; 0/Interleukin-6; 11016-17-4/Phycoerythrin; EC 3.1.3.48/Antigens, CD45 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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