| Flow cytometric characterization of the response of Fanconi's anemia cells to mitomycin C treatment. | |
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MedLine Citation:
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PMID: 6804195 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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DNA flow histogram analysis, using 33342 Hoechst as a stain, has been used to detect the effect of the potentially bifunctional alkylating agent, mitomycin C (MMC) on dermal fibroblasts from patients with Fanconi's anemia (FA), a hereditary human disease characterized by pancytopenia, hypersensitivity to DNA-crosslinking agents, congenital abnormalities and a predisposition for neoplasia. At 24 or 48 hr after a 2-hr exposure to 0.05 or 0.10 micrograms/ml MMC, (3)HdT incorporation was reduced to a greater extent in FA cells than in normal cells. Cells sorted from the last half of S phase showed a slightly greater inhibition of (3)HdT incorporation than did those sorted from the first half of S. Fanconi's anemia cells exhibited a marked accumulation in the G(2) + M peak of flow histograms following exposure to MMC. Twenty-four hr after treatment with .0.5 micrograms/ml MMC, the G(2) + M fraction of FA cells (eight lines) increased to more than 0.5 from a control value of approximately 0.02. Both normals (six lines) and heterozygotes (eight lines) showed, on the average, much less of a G(2) + M increment than did FA cells, even after exposure to 0.1 micrograms/ml MMC. Examination of cells sorted from the G(2) + M peak revealed that MMC-treated FA cells were blocked prior to mitosis. To determine whether the response of FA cells was specific for bifunctional alkylating agent, cells were also treated with ethylmethanesulfonate, a monofunctional agent. Twenty-four hours after exposure to 0.25 or 0.5 mg/ml ethylmethanesulfonate, FA and normal cells showed similar, small increases in the G(2) + M peak. The results suggest the utility of flow cytometry in the diagnostic evaluation of fibroblasts from patients suspected of having Fanconi's anemia. |
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Authors:
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T N Kaiser; A Lojewski; C Dougherty; L Juergens; E Sahar; S A Latt |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cytometry Volume: 2 ISSN: 0196-4763 ISO Abbreviation: Cytometry Publication Date: 1982 Mar |
Date Detail:
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Created Date: 1982-07-19 Completed Date: 1982-07-19 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8102328 Medline TA: Cytometry Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 291-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alkylating Agents
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pharmacology Anemia, Aplastic / genetics* Cell Cycle DNA Repair* DNA Replication / drug effects Fanconi Anemia / genetics* Flow Cytometry Heterozygote Humans Mitomycin Mitomycins / pharmacology* Mitosis / drug effects |
| Grant Support | |
ID/Acronym/Agency:
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GM21121/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Alkylating Agents; 0/Mitomycins; 50-07-7/Mitomycin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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