| Flow cytometric cell-cycle analysis of cultured fibroblasts from the giant panda, Ailuropoda melanoleuca L. | |
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MedLine Citation:
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PMID: 12788051 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In animal cloning, it is generally believed that the inactive diploid G(0)or G(1)stage of the cell cycle is beneficial to initiate cell-cycle coordination and reprogramming following transfer of the donor nucleus. Previous experiments have demonstrated that serum starvation results in quiescent cell stage. Some experiments show that the majority of cells in a fully confluent cell culture are also in an inactive G(1)stage. In order to provide more G(0)/G(1)stage cells for giant panda cloning, we carried out a flow cytometric analysis of the cell cycle of fibroblasts from the abdominal muscle of a giant panda at different passage numbers under different growth conditions, and after different periods of serum starvation. The percentage of G(0)+G(1)stage cells differed significantly under different growth conditions. Serum starvation effectively increased the percentage of G(0)+G(1)stage cells, and the cell cycle characteristics following serum starvation for varying periods of time differed with this and the initial confluency of the cultures. The data should help in choosing the optimal stage for preparing donor cells as well as increasing the potential cloning efficiency in our study of giant panda cloning. |
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Authors:
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Zhi-Ming Han; Da-Yuan Chen; Jin-Song Li; Qing-Yuan Sun; Peng-Yan Wang; Jun Du; He-Min Zhang |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell biology international Volume: 27 ISSN: 1065-6995 ISO Abbreviation: Cell Biol. Int. Publication Date: 2003 |
Date Detail:
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Created Date: 2003-06-05 Completed Date: 2003-12-01 Revised Date: 2009-11-03 |
Medline Journal Info:
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Nlm Unique ID: 9307129 Medline TA: Cell Biol Int Country: England |
Other Details:
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Languages: eng Pagination: 349-53 Citation Subset: IM |
Affiliation:
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State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Cycle* / drug effects Cells, Cultured Culture Media, Serum-Free / pharmacology Female Fibroblasts / cytology*, drug effects Flow Cytometry / methods* Ursidae* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media, Serum-Free |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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