| Flow-cytometric analysis of testes in infertile men: a comparison of the ploidy to routine histopathologic study. | |
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MedLine Citation:
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PMID: 1743229 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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DNA flow-cytometric analysis was performed on the testicular needle biopsies of 25 infertile men with azoospermia or oligozoospermia to evaluate the ability of DNA histograms in order to detect and quantify alterations in spermatogenesis. Concomitant histopathologic study was performed on the tissues from needle biopsy. In contrast to difficulty in quantifying spermatogenesis in histopathologic examination, flow-cytometric analysis revealed characteristic ploidy patterns in the relative proportions of haploid (1 n), diploid (2 n) and tetraploid (4 n) cells corresponding to the histopathologic appearances of normal spermatogenesis, hypospermatogenesis, maturation arrest and aspermatogenesis. Findings evaluated with flow cytometry were well correlated with those from routine histopathologic study. In 21 of these patients (84%) there was concordance between histopathologic and flow-cytometric diagnoses. However, in 4 patients (16%) there was discordance between two diagnostic modalities. In conclusion, DNA flow cytometry of testicular biopsies was a reproducible, objective and quantitative approach in evaluating infertile men, and it is a promising method to investigate spermatogenesis in an outpatient clinic in lieu of formal testicular histopathologic study. |
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Authors:
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S E Lee; M S Choo |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European urology Volume: 20 ISSN: 0302-2838 ISO Abbreviation: Eur. Urol. Publication Date: 1991 |
Date Detail:
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Created Date: 1992-01-16 Completed Date: 1992-01-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7512719 Medline TA: Eur Urol Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 33-8 Citation Subset: IM |
Affiliation:
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Department of Urology, Seoul National University College of Medicine, Korea. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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DNA
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analysis* Flow Cytometry* Humans Infertility, Male / genetics*, pathology, physiopathology Male Ploidies Spermatogenesis Testis / pathology* |
| Chemical | |
Reg. No./Substance:
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9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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