| Flow cytometric analysis of peripheral blood dendritic cells in patients with severe sepsis. | |
| | |
MedLine Citation:
|
PMID: 20687203 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
BACKGROUND: Dendritic cells (DC) play a key role in cell-mediated immunity. We aimed to analyse the number and function of peripheral blood (PB) myeloid and plasmacytoid DC (mDC/pDC) in patients with severe sepsis. METHODS: Twenty-six septic patients, 20 surgical patients (abdominal aortic aneurysm) and 20 healthy controls were enrolled in this prospective study. RESULTS: At day 1 (enrollment in the study), septic patients showed in comparison with healthy controls, decreased mDC (P < 0.001) and increased pDC (P = 0.03), resulting in a reduction of the mDC/pDC ratio (P < 0.001). Surgery induced a decrease in both mDCs and pDCs level, without modification of mDC/pDC ratio. Septic patients included 15 survivors and 11 nonsurvivors. At day 1 no significant difference was found in mDC between the two groups, while pDCs were significantly higher in nonsurvivors (P = 0.03). At the outcome, mDC were selectively increased with respect to day 1 in survivors (P = 0.001), while no significant differences were observed as far as pDC count is concerned in both groups. Sorted DC from septic patients showed in comparison with healthy controls, reduced levels of HLA DR (P < 0.001), CD11c (P < 0.001), CD83 (P = 0.006) and of costimulatory molecule CD86 (P = 0.003); an up-regulation of chemokine receptor CXCR4 (P = 0.031) and increased apoptosis (P < 0.001). CONCLUSIONS: Sepsis has a profound effect on PB DC compartment. These alterations appear to be sepsis-specific. Increased apoptosis and alteration of migration and trafficking mechanism may be involved in DC compartment modification. DC alterations could contribute to sepsis-mediated immunoparalysis. |
| | |
Authors:
|
Francesca Riccardi; Matteo G Della Porta; Bianca Rovati; Alberto Casazza; Danila Radolovich; Mara De Amici; Marco Danova; Martin Langer |
Related Documents
:
|
15630253 - Antitumor activity and action mechanisms of lactobacillus casei through the regulation ... 15265893 - Cpg-c immunostimulatory oligodeoxyribonucleotide activation of plasmacytoid dendritic c... 10672833 - Apoptosis of human gastric epithelial cells via caspase-3 activation in response to hel... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Cytometry. Part B, Clinical cytometry Volume: 80 ISSN: 1552-4957 ISO Abbreviation: Cytometry B Clin Cytom Publication Date: 2011 Jan |
Date Detail:
|
Created Date: 2010-12-24 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101235690 Medline TA: Cytometry B Clin Cytom Country: United States |
Other Details:
|
Languages: eng Pagination: 14-21 Citation Subset: IM |
Copyright Information:
|
Copyright © 2010 International Clinical Cytometry Society. |
Affiliation:
|
Servizio Anestesia e Rianimazione II, IRCCS Policlinico, S. Matteo and University of Pavia, Pavia, Italy. francesca_riccardi@libero.it |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Evaluation of degranulation and cytokine production in natural killer cells from spondyloarthritis p...
Next Document: Etiologies of spontaneous pneumomediastinum in children in middle Taiwan.