| Flow cytometric analysis of granulosa cells from developing rat follicles. | |
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MedLine Citation:
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PMID: 1849576 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Immature rats were treated with diethylstilboestrol (DES) or pregnant mares' serum gonadotropin (PMSG) and forward angle light-scatter (FALS) and 90 degrees light-scatter (90 degrees LS) signals were used to measure the size and the granularity (internal organization) of the granulosa cells, respectively. The results confirmed the presence of two major populations of granulosa cells in the ovaries of both groups of rats, with the same percentage of larger cells in both treatments (52.3% in DES, 49.5% in PMSG). Since DES treatment brings about granulosa cell growth while PMSG treatment causes growth and differentiation, it is evident that there is heterogeneity in granulosa cell sizes during different states of growth and differentiation. There was also heterogeneity in sizes of granulosa cells harvested from follicles of small (less than 210 microns), medium (210-420 microns) and large (greater than 420 microns) diameter. Quadrant analysis of granulosa cells in various fractions collected from Percoll gradients suggested an increase in granularity in the small and large granulosa cell populations. Cell cycle analysis of small and large granulosa cell populations collected from large follicles of rats treated with PMSG indicated that each population was distributed in G0/G1, S and G2/M phases. These results demonstrate that populations of small and large granulosa cells exist in rat ovarian follicles during various stages of growth and differentiation. |
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Authors:
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I M Rao; W C Allsbrook; B A Conway; J E Martinez; J R Beck; C G Pantazis; T M Mills; E Anderson; V B Mahesh |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of reproduction and fertility Volume: 91 ISSN: 0022-4251 ISO Abbreviation: J. Reprod. Fertil. Publication Date: 1991 Mar |
Date Detail:
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Created Date: 1991-05-15 Completed Date: 1991-05-15 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0376367 Medline TA: J Reprod Fertil Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 521-30 Citation Subset: IM |
Affiliation:
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Department of Physiology and Endocrinology, Medical College of Georgia, Augusta 30912. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Cycle* Cell Separation Cell Survival / drug effects Centrifugation, Density Gradient Diethylstilbestrol / pharmacology Female Flow Cytometry* / methods Gonadotropins, Equine / pharmacology Granulosa Cells / cytology*, drug effects Ovarian Follicle / growth & development Povidone Rats Rats, Inbred Strains Scattering, Radiation Silicon Dioxide |
| Grant Support | |
ID/Acronym/Agency:
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HD24488/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Gonadotropins, Equine; 56-53-1/Diethylstilbestrol; 65455-52-9/Percoll; 7631-86-9/Silicon Dioxide; 9003-39-8/Povidone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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