| Flow cytometric analysis of the contributing factors for antimicrobial activity enhancement of cell-penetrating type peptides: case study on engineered apidaecins. | |
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MedLine Citation:
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PMID: 20302844 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Contributing factors for the antimicrobial activity enhancement of N-terminally engineered mutants of cell-penetrating apidaecins were analyzed based on their cell-penetration efficiency. The flow cytometric analysis of the engineered apidaecins labeled with carboxyfluorescein (FAM) revealed their enhanced cell-penetrating efficiencies into Escherichia coli that should be one of key factors causing the enhanced antimicrobial activity. It is noteworthy that, for one mutant, the enhancement in antimicrobial activity (18-fold higher than wild type) was greater than that of cell penetration (5.9-fold), suggesting that the N-terminal mutation may reinforce both interaction with unidentified intracellular target(s) and cell-penetration efficiency. |
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Authors:
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Ken'ichiro Matsumoto; Yoshitake Orikasa; Kenta Ichinohe; Shigeki Hashimoto; Toshihiko Ooi; Seiichi Taguchi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-17 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 395 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-04-26 Completed Date: 2010-05-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 7-10 Citation Subset: IM |
Copyright Information:
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2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13W8, Kita-ku, Sapporo 060-8628, Japan. mken@eng.hokudai.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Antimicrobial Cationic Peptides / genetics, metabolism*, pharmacology* Escherichia coli / drug effects, metabolism Flow Cytometry / methods* Fluoresceins / chemistry, metabolism Fluorescent Dyes / chemistry, metabolism Molecular Sequence Data Protein Engineering* Protein Transport |
| Chemical | |
Reg. No./Substance:
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0/Antimicrobial Cationic Peptides; 0/Fluoresceins; 0/Fluorescent Dyes; 123997-21-7/apidaecin; 3301-79-9/6-carboxyfluorescein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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