Document Detail

Flow cytometric analysis of the contributing factors for antimicrobial activity enhancement of cell-penetrating type peptides: case study on engineered apidaecins.
MedLine Citation:
PMID:  20302844     Owner:  NLM     Status:  MEDLINE    
Contributing factors for the antimicrobial activity enhancement of N-terminally engineered mutants of cell-penetrating apidaecins were analyzed based on their cell-penetration efficiency. The flow cytometric analysis of the engineered apidaecins labeled with carboxyfluorescein (FAM) revealed their enhanced cell-penetrating efficiencies into Escherichia coli that should be one of key factors causing the enhanced antimicrobial activity. It is noteworthy that, for one mutant, the enhancement in antimicrobial activity (18-fold higher than wild type) was greater than that of cell penetration (5.9-fold), suggesting that the N-terminal mutation may reinforce both interaction with unidentified intracellular target(s) and cell-penetration efficiency.
Ken'ichiro Matsumoto; Yoshitake Orikasa; Kenta Ichinohe; Shigeki Hashimoto; Toshihiko Ooi; Seiichi Taguchi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-17
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  395     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-26     Completed Date:  2010-05-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7-10     Citation Subset:  IM    
Copyright Information:
2010 Elsevier Inc. All rights reserved.
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13W8, Kita-ku, Sapporo 060-8628, Japan.
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MeSH Terms
Amino Acid Sequence
Antimicrobial Cationic Peptides / genetics,  metabolism*,  pharmacology*
Escherichia coli / drug effects,  metabolism
Flow Cytometry / methods*
Fluoresceins / chemistry,  metabolism
Fluorescent Dyes / chemistry,  metabolism
Molecular Sequence Data
Protein Engineering*
Protein Transport
Reg. No./Substance:
0/Antimicrobial Cationic Peptides; 0/Fluoresceins; 0/Fluorescent Dyes; 123997-21-7/apidaecin; 3301-79-9/6-carboxyfluorescein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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