| Flow cytometric analysis of benthic prokaryotes attached to sediment particles. | |
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MedLine Citation:
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PMID: 19751775 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Appropriate detachment treatments are required to analyze prokaryotes associated with streambed sediments by flow cytometry. Using our previously optimized protocol, two groups of cells exhibiting different nucleic acid contents were easily detectable. However, the Nucleic Acid Double Staining assay proved that detachment procedures negatively affect the cell membrane integrity. |
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Authors:
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Stefano Amalfitano; Stefano Fazi Alberto Puddu |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2009-09-13 |
Journal Detail:
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Title: Journal of microbiological methods Volume: 79 ISSN: 1872-8359 ISO Abbreviation: J. Microbiol. Methods Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2009-10-20 Completed Date: 2009-12-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8306883 Medline TA: J Microbiol Methods Country: Netherlands |
Other Details:
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Languages: eng Pagination: 246-9 Citation Subset: IM |
Affiliation:
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Water Research Institute (IRSA-CNR), Via Salaria Km 29.300, Monterotondo, Rome, Italy. amalfitano@irsa.cnr.it |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Flow Cytometry
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methods* Geologic Sediments / microbiology* Nucleic Acids / analysis, genetics Prokaryotic Cells / chemistry, classification* Rivers* Staining and Labeling / methods |
| Chemical | |
Reg. No./Substance:
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0/Nucleic Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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