Document Detail


Flow cytometric DNA analysis after immunoselection of bladder tumor cells with monoclonal antibody DU83.21.
MedLine Citation:
PMID:  1698117     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Adequate preservation of neoplastic cells and the elimination of interference by inflammatory cells in measuring tumor cell DNA content represent two important objectives necessary for accurate flow cytometric analysis of bladder carcinomas. An experimental model consisting of a mixture of cultured bladder carcinoma cells (T24) and human buffy-coat (BC) cells was used to evaluate various preservatives and an anti-bladder carcinoma monoclonal antibody (MoAb), DU83.21, for separating inflammatory cells (BC cells) from T24 cells. A final concentration of 25% ethanol was found to be the most effective preservative of several tested. After incubation with the MoAb DU83.21 and propidium iodide (DNA stain), the T24 cells could be separated from the BC cells, permitting accurate DNA analysis of the tumor cells. Application of this system to specimens from bladder cancer patients enhanced the detection and DNA analysis of the tumor cell populations.
Authors:
G L Wright; J P Alexander; A M Konchuba; P F Schellhammer; S M Schlossberg
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer     Volume:  66     ISSN:  0008-543X     ISO Abbreviation:  Cancer     Publication Date:  1990 Sep 
Date Detail:
Created Date:  1990-10-19     Completed Date:  1990-10-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0374236     Medline TA:  Cancer     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1242-52     Citation Subset:  AIM; IM    
Affiliation:
Department of Microbiology and Immunology, Eastern Virginia Medical School, Norfolk 23501.
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MeSH Terms
Descriptor/Qualifier:
Aneuploidy
Antibodies, Monoclonal
Blood Cells / pathology
Carcinoma, Transitional Cell / analysis,  genetics*,  pathology
Cell Line
Cell Separation
DNA, Neoplasm / analysis*
Ethanol
Flow Cytometry / methods*
Fluorescent Antibody Technique
Humans
Staining and Labeling
Urinary Bladder / pathology
Urinary Bladder Neoplasms / analysis,  genetics*,  pathology
Grant Support
ID/Acronym/Agency:
CA-26659/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/DNA, Neoplasm; 64-17-5/Ethanol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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