| Flow cytofluorimetric determination of protein distribution throughout the cell cycle. | |
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MedLine Citation:
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PMID: 2670486 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Quantitative treatment of multiparametric determination on cells using flow cytofluorometry was made possible by the development of a computer program that allows the relative quantification of a specific protein as a function of the position of cells during the cell cycle. This type of analysis provides interesting information about the distribution of a given protein throughout the cell cycle. Four examples showing the distribution of specific proteins illustrate such a quantification during the cell cycle in two different cell lines. The program also allows for the handling of a series of histograms obtained by the analysis of protein distribution as a function of DNA content in relation to a third parameter. To illustrate possible applications for this program, the evolution of the distribution of two proteins, the oncoprotein p53 and the histone H1(0), during the induced differentiation of murine erythroleukemia cells has been studied. |
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Authors:
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S Khochbin; A Chabanas; P Albert; J J Lawrence |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cytometry Volume: 10 ISSN: 0196-4763 ISO Abbreviation: Cytometry Publication Date: 1989 Jul |
Date Detail:
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Created Date: 1989-10-11 Completed Date: 1989-10-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8102328 Medline TA: Cytometry Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 484-9 Citation Subset: IM |
Affiliation:
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Departement de Recherche Fondamentale, Unité Inserm 309, CEN-Grenoble, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Cycle* Cell Differentiation DNA, Neoplasm / analysis* Histones / metabolism* Immunohistochemistry / methods* Mice Neoplasm Proteins / metabolism* Phosphoproteins / metabolism* Software Tumor Cells, Cultured / chemistry*, cytology Tumor Suppressor Protein p53 |
| Chemical | |
Reg. No./Substance:
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0/DNA, Neoplasm; 0/Histones; 0/Neoplasm Proteins; 0/Phosphoproteins; 0/Tumor Suppressor Protein p53 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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