| Flow cytochemical patterns of white blood cells in human haematopoietic malignancies. II. Chronic leukaemias. | |
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MedLine Citation:
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PMID: 3479189 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Peripheral blood samples from 73 patients with chronic leukaemia were measured with the Technicon H-6000 automated haematology analyser to provide flow cytochemical (peroxidase content) and volume (light scatter) discriminated scattergram patterns. For chronic granulocytic leukaemia (CGL), these patterns were so reproducible and distinct that they allowed an immediate diagnosis even without the benefit of microscopic examination. Relative and absolute basophilia was an invariable feature, and remained detected by the H-6000 even when the patient was in haematologic and cytogenetic remission or progressed into blast crisis (BC). Most patients in BC also demonstrated an inordinately high number of large unstained cells (LUC) and high proportions of 'lymphocytes' (small blasts with no peroxidase content by visual inspection). Thus, for patients with CGL, LUC values above 10%, and/or steady increments in the proportion of 'lymphocytes', merit concern as these changes may herald an accelerated phase of disease. The scattergram pattern of untreated chronic lymphocytic leukaemia (CLL) showed a dense accumulation of data points within the lymphocytic 'box' with a small cluster of granulocytic elements. Most patients also had a frankly abnormal proportion of LUC. Sixteen patients with CLL were compared for ratios of LUC to lymphocytes and stage of disease; patients with the most advanced stage (IV) had the highest, statistically significant values, than the patients with more benign disease. Thus, it is possible that follow up with this instrument of patients with CLL will also allow early detection of an impending prolymphocytoid transformation (accelerated phase) of this disease. |
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Authors:
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B Drewinko; P Bollinger; C Brailas; J Wyatt; E Simson; J M Trujillo |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: British journal of haematology Volume: 67 ISSN: 0007-1048 ISO Abbreviation: Br. J. Haematol. Publication Date: 1987 Oct |
Date Detail:
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Created Date: 1988-01-14 Completed Date: 1988-01-14 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372544 Medline TA: Br J Haematol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 157-65 Citation Subset: IM |
Affiliation:
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Division of Laboratory Medicine, University of Texas System Cancer Center, M.D. Anderson Hospital and Tumor Institute at Houston 77030. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Separation Flow Cytometry* Humans Leukemia, Lymphoid / blood* Leukemia, Myeloid / blood* Leukocyte Count Lymphocytes* |
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