| Flow-assisted allergy diagnosis: current applications and future perspectives. | |
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MedLine Citation:
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PMID: 16918504 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Physicians predominantly rely upon quantification of serum-specific immunoglobulin E (IgE) and/or skin test to confirm clinically suspected IgE-mediated allergy. However, for various reasons, identification of the offending allergen(s) and potentially cross-reactive structures is not always straightforward. Flow-assisted allergy diagnosis relies upon quantification of alterations in the expression of particular basophilic activation markers. Actually, upon challenge with a specific allergen, basophils not only secrete quantifiable bioactive mediators but also upregulate the expression of different markers which can be detected efficiently by flow cytometry using specific monoclonal antibodies. Currently, the technique has been applied in the investigation of IgE-mediated allergy caused by classical inhalant allergens, food, Hevea latex, hymenoptera venoms and drugs. It is also appreciated; the technique proves valuable in the diagnosis of non-IgE-mediated (anaphylactoid) reactions such drug hypersensitivity and the detection of autoantibodies in certain forms of chronic urticaria. This review will not address immunologic features, characteristics and general pitfalls of flow-assisted analysis of in vitro-activated basophils as summarized elsewhere. After a recapitulation of the principles and some specific technical issues of flow-assisted analysis of in vitro-activated basophils, we principally focus on the current clinical and research applications of the basophil activation tests. Personal experience of both research groups is provided, where appropriate. Finally, a viewpoint on how the field might evolve in the following years is provided. |
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Authors:
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D G Ebo; J Sainte-Laudy; C H Bridts; C H Mertens; M M Hagendorens; A J Schuerwegh; L S De Clerck; W J Stevens |
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Publication Detail:
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Type: Journal Article; Review |
Journal Detail:
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Title: Allergy Volume: 61 ISSN: 0105-4538 ISO Abbreviation: Allergy Publication Date: 2006 Sep |
Date Detail:
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Created Date: 2006-08-21 Completed Date: 2007-01-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7804028 Medline TA: Allergy Country: Denmark |
Other Details:
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Languages: eng Pagination: 1028-39 Citation Subset: IM |
Affiliation:
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Department of Immunology, Allergology, Rheumatology, University Antwerp (UA), Antwerpen, Belgium. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Separation* Flow Cytometry* / trends Forecasting Humans Hypersensitivity / diagnosis* |
| Comments/Corrections | |
Comment In:
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Allergy. 2006 Sep;61(9):1025-7
[PMID:
16918503
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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