Document Detail

Flow-PRA evaluation for antibody screening in patients awaiting kidney transplantation.
MedLine Citation:
PMID:  11005318     Owner:  NLM     Status:  MEDLINE    
Flow-PRA is a flow cytometric method for both anti-HLA class I and class II antibody (Ab) detection. We evaluated this technique for Ab screening in patients awaiting kidney transplantation. After having established a rigorous threshold for positivity, a three-dilution difference in sensitivity between Flow-PRA and complement-dependent cytotoxicity (CDC) persisted. The sensitivity of the method was satisfactory since all CDC-positive sera were also found to be positive with the Flow-PRA method. Discrimination between anti-HLA class I and class II Abs was excellent. Furthermore, all sera responsible for a positive flow cytometry crossmatch (FCXM) and a negative CDC-crossmatch (CDCXM) at the time of a putative transplant were found to be positive with Flow-PRA beads. The specificity was excellent for anti-class I Ab detection since no false positive serum was found. On the other hand, the specificity was lower for anti-class II detection, since 8.3% (2/24) false positive results were detected among all the negative sera tested. Overall, our results suggested that Flow-PRA should be of value for anti-HLA Ab screening prior to kidney transplantation.
J M Rebibou; J Chabod; M C Bittencourt; C Thevénin; J M Chalopin; P Hervé; P Tiberghien
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Transplant immunology     Volume:  8     ISSN:  0966-3274     ISO Abbreviation:  Transpl. Immunol.     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2001-01-11     Completed Date:  2001-01-11     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9309923     Medline TA:  Transpl Immunol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  125-8     Citation Subset:  IM    
Laboratory of Immunogenetics, EFS de Bourgogne Franche-Comté, Besançon, France.
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MeSH Terms
Flow Cytometry*
Histocompatibility Antigens Class I / immunology*
Histocompatibility Antigens Class II / immunology*
Histocompatibility Testing
Isoantibodies / blood*
Kidney Transplantation*
Reproducibility of Results
Reg. No./Substance:
0/Histocompatibility Antigens Class I; 0/Histocompatibility Antigens Class II; 0/Isoantibodies

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