Document Detail


Flow cytometry for gene expression studies in Mammalian spermatogenesis.
MedLine Citation:
PMID:  20389037     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mammalian spermatogenesis is nowadays still poorly understood at the molecular level, mainly due to the heterogeneous nature of testes, which contain a high number of different cell types, and to the lack of spermatogenic cell culture systems for in vitro studies. As a consequence, the development and/or application of methodological approaches aiming at the enrichment or purification of specific testicular cell types are of great interest and have been addressed by scientists for at least 4 decades. Among the many applications that flow cytometry (FC) has gained since its invention, analysis and sorting of spermatogenic cell populations represent a promising strategy to efficiently overcome testis heterogeneity drawback. Surprisingly, FC has been only rarely used as a preparative method for downstream gene expression studies in specific spermatogenic stages. This work aims to provide an overview of FC for spermatogenic studies including preparation of testicular single cell suspensions, dyes for DNA staining, and our own experience with rodent testis material.
Authors:
A Geisinger; R Rodr?guez-Casuriaga
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Publication Detail:
Type:  Journal Article; Review     Date:  2010-04-09
Journal Detail:
Title:  Cytogenetic and genome research     Volume:  128     ISSN:  1424-859X     ISO Abbreviation:  Cytogenet. Genome Res.     Publication Date:  2010  
Date Detail:
Created Date:  2010-06-04     Completed Date:  2010-06-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101142708     Medline TA:  Cytogenet Genome Res     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  46-56     Citation Subset:  IM    
Copyright Information:
Copyright 2010 S. Karger AG, Basel.
Affiliation:
Secci?n Bioqu?mica, Facultad de Ciencias, Instituto de Investigaciones Biol?gicas Clemente Estable, Montevideo 11600 , Uruguay. geisinge@iibce.edu.uy
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MeSH Terms
Descriptor/Qualifier:
Animals
Flow Cytometry / methods*
Gene Expression*
Male
Spermatogenesis*
Testis / metabolism*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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