| A flow cytometric assay to quantify in vivo bacterial uptake by alveolar macrophages. | |
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MedLine Citation:
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PMID: 20171249 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Our laboratory has developed a flow cytometric assay to quantify alveolar macrophage (Mcapital EF, Cyrillic) phagocytosis of bacteria within a live animal. Mcapital EF, Cyrillics collected by bronchoalveolar lavage from rats infected transtracheally with Syto 9-labeled bacteria are fluorescently labeled for identification and analyzed by flow cytometry to quantify their bacterial uptake. |
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Authors:
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Adam M Pitz; Greg A Perry; Heather C Jensen-Smith; Martha J Gentry-Nielsen |
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Publication Detail:
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Type: Evaluation Studies; Journal Article Date: 2010-02-18 |
Journal Detail:
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Title: Journal of microbiological methods Volume: 81 ISSN: 1872-8359 ISO Abbreviation: J. Microbiol. Methods Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-04-20 Completed Date: 2010-07-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8306883 Medline TA: J Microbiol Methods Country: Netherlands |
Other Details:
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Languages: eng Pagination: 194-6 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Microbiology and Immunology, Creighton University School of Medicine, 2500 California Plaza, Omaha, NE 68178, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bacteria / immunology* Bronchoalveolar Lavage Flow Cytometry / methods* Fluorescent Dyes / pharmacology Lung / immunology Macrophages, Alveolar / microbiology* Male Organic Chemicals / pharmacology Phagocytosis* Rats Rats, Sprague-Dawley Staining and Labeling |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 0/Organic Chemicals; 0/SYTO 9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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