Document Detail

Flotillin-2 expression in the human gut: from a cell model to human tissue in health and inflammatory bowel diseases.
MedLine Citation:
PMID:  23983584     Owner:  NLM     Status:  MEDLINE    
BACKGROUND AND AIMS: The etiopathogenesis of inflammatory bowel diseases (IBD) remains largely unexplained. Flotillins (flotillin-1 and flotillin-2) are ubiquitous proteins which have been linked to inflammation and regeneration. We hypothesized that alterations in the expression of flotillin-2 in enterocytes may be related to the pathogenesis of IBD as a classical example of an inflammatory disorder of mostly unknown origin.
METHODS: Cell and tissue localization of flotillin-2 (and -1) were investigated by immunofluorescent staining in 1. polarized and unpolarized CaCo-2w cells as a model of human enterocytes (native and after TNFα stimulation) and 2. intestinal biopsies from controls, patients with ulcerative colitis (UC) and patients with Crohn's disease (CD). For quantification of flotillin-2, we analyzed its expression in ileal and colonic biopsies from controls, UC patients and CD patients using real-time RT-PCR, Western blot and indirect immunofluorescence.
RESULTS: In polarized CaCo-2w cells and human enterocytes in biopsies, flotillins were localized at the basolateral membrane and on subapical vesicles, but not in the apical membrane. Flotillin-2 expression did not differ between UC patients, CD patients and controls. However, it was significantly higher in colonic biopsies compared to ileal biopsies in all groups.
CONCLUSIONS: By virtue of its abundant expression in enterocytes, flotillin-2 must have an essential function in intestinal physiology, especially in the colon. Yet our data could not link flotillin-2 to the pathogenesis of IBD.
Annika Gauss; Inga Buchholz; Alexandra Zahn; Gerd Schmitz; Wolfgang Stremmel; Joachim Fuellekrug; Robert Ehehalt
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Publication Detail:
Type:  In Vitro; Journal Article     Date:  2013-08-03
Journal Detail:
Title:  International journal of medical sciences     Volume:  10     ISSN:  1449-1907     ISO Abbreviation:  Int J Med Sci     Publication Date:  2013  
Date Detail:
Created Date:  2013-08-28     Completed Date:  2014-03-17     Revised Date:  2014-08-26    
Medline Journal Info:
Nlm Unique ID:  101213954     Medline TA:  Int J Med Sci     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  1259-70     Citation Subset:  IM    
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MeSH Terms
Caco-2 Cells
Colitis, Ulcerative / metabolism
Crohn Disease / metabolism
Inflammatory Bowel Diseases / metabolism
Membrane Microdomains / metabolism
Membrane Proteins / metabolism*
Middle Aged
Reg. No./Substance:
0/Membrane Proteins; 0/flotillins

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