Document Detail

Flotillas of lipid rafts in transit amplifying cell-like keratinocytes.
MedLine Citation:
PMID:  12925211     Owner:  NLM     Status:  MEDLINE    
Lipid rafts are dynamic membrane microdomains enriched in cholesterol and sphingolipids and are involved in the regulation of a variety of cellular processes, such as proliferation, apoptosis and cell motility. We have previously described that large lipid raft aggregates are readily detectable on cultured keratinocyte cell line HaCaT by staining with the fluorescein-tagged cholera toxin (CTx-FITC). In this paper we adopted this method for the detection of lipid rafts in human epidermis and keratinocytes in culture. Double labelling of showed the non-overlapping clusters of basal cells in human epidermis: CD29dimCTx-FITCbright cells in the deep rate ridges and CD29brightCTx-FITCdim cells at the tips of dermal papillae. A similar patchy, non-overlapping staining pattern was observed in cultured keratinocytes in vitro. CTx-FITCbright cells are mitotically active whereas a large proportion of CTx-FITCdim cells are quiescent. We conclude that the epidermal stem-like cells, previously shown to occupy the tips of dermal papillae and to exhibit high density of membrane beta1 integrin have a low content of lipid rafts. In contrast, the putative transit amplifying cells in deep rate ridges show enrichment in lipid rafts. Thus, lipid rafts may be a factor controlling the mitotic activity of epidermal keratinocytes and possibly the differentiation of stem cells into the transit amplifying cells.
Robert Gniadecki; Bo Bang
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of investigative dermatology     Volume:  121     ISSN:  0022-202X     ISO Abbreviation:  J. Invest. Dermatol.     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-08-19     Completed Date:  2003-10-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0426720     Medline TA:  J Invest Dermatol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  522-8     Citation Subset:  IM    
Department of Dermatology D92, Bispebjerg Hospital, Bispebjerg bake 23, University of Copenhagen, DK-2400 Copenhagen NV, Denmark.
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MeSH Terms
Cell Division / physiology
Cell Membrane / physiology
Cholera Toxin
Epidermis / cytology
Fluorescent Dyes
Keratinocytes / cytology*,  physiology*
Membrane Microdomains / physiology*
Mitosis / physiology
Stem Cells / cytology
Reg. No./Substance:
0/Detergents; 0/Fluorescent Dyes; 3326-32-7/Fluorescein-5-isothiocyanate; 9012-63-9/Cholera Toxin

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