Document Detail


Flexible peritoneal windows for quantitative fluorescence and bioluminescence preclinical imaging.
MedLine Citation:
PMID:  23348789     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
AbstractAt present, there is considerable interest in the use of in vivo fluorescence and bioluminescence imaging to track the onset and progression of pathologic processes in preclinical models of human disease. Optical quantitation of such phenomena, however, is often problematic, frequently complicated by the overlying tissue's scattering and absorption of light, as well as the presence of endogenous cutaneous and subcutaneous fluorophores. To partially circumvent this information loss, we report here the development of flexible, surgically implanted, transparent windows that enhance quantitative in vivo fluorescence and bioluminescence imaging of optical reporters. These windows are metal and glass free and thus compatible with computed tomography, magnetic resonance imaging, positron emission tomography, and single-photon emission computed tomography; they also permit visualization of much larger areas with fewer impediments to animal locomotion and grooming than those previously described. To evaluate their utility in preclinical imaging, we surgically implanted these windows in the abdominal walls of female athymic nude mice and subsequently inoculated each animal with 1 × 104 to 1 × 106 bioluminescent human ovarian cancer cells (SKOV3ip.1-luc). Longitudinal imaging studies of fenestrated animals revealed up to 48-fold gains in imaging sensitivity relative to nonfenestrated animals, with relatively few complications, allowing wide-field in vivo visualization of nascent metastatic ovarian cancer colonization.
Authors:
Jeffrey S Souris; Jonathan A Hickson; Lambda Msezane; Carrie W Rinker-Schaeffer; Chin-Tu Chen
Related Documents :
15123919 - A comparison of digital cameras: features essential for the orthopaedic surgeon.
20134839 - Removal of instrument signature from mariner 9 television images of mars.
2769409 - Phantom feet on digital radionuclide images and other scary computer tales.
24239209 - Diagnostic performance of narrowed spectrum endoscopy, autofluorescence imaging, and co...
12462719 - A novel silicon array designed for intraoperative charged particle imaging.
7411219 - Misalignment of multiple photopeak analyzer outputs: effects on imaging. concise commun...
25240319 - A multilayer ontology of instruments for neurological, behavioral and cognitive assessm...
17282919 - On the small vessel detection in high resolution retinal images.
22918759 - "click"-cyclized (68)ga-labeled peptides for molecular imaging and therapy: synthesis a...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular imaging     Volume:  12     ISSN:  1536-0121     ISO Abbreviation:  Mol Imaging     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101120118     Medline TA:  Mol Imaging     Country:  United States    
Other Details:
Languages:  eng     Pagination:  28-38     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Development and characterization of 89Zr-labeled panitumumab for immuno-positron emission tomographi...
Next Document:  Monitoring Glioma Growth and Tumor Necrosis with the U-SPECT-II/CT Scanner by Targeting Integrin ?v?...