Document Detail


Flexible microelectrode arrays to interface epicardial electrical signals with intracardial calcium transients in zebrafish hearts.
MedLine Citation:
PMID:  22124886     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The zebrafish (Danio rerio) is an emerging genetic model for regenerative medicine. In humans, myocardial infarction results in the irreversible loss of cardiomyocytes. However, zebrafish hearts fully regenerate after a 20% ventricular resection, without either scarring or arrhythmias. To study this cardiac regeneration, we developed implantable flexible multi-microelectrode membrane arrays that measure the epicardial electrocardiogram signals of zebrafish in real-time. The microelectrode electrical signals allowed for a high level of both temporal and spatial resolution (~20 μm), and the signal to noise ratio of the epicardial ECG was comparable to that of surface electrode ECG (7.1 dB vs. 7.4 dB, respectively). Processing and analysis of the signals from the microelectrode array demonstrated distinct ECG signals: namely, atrial conduction (P waves), ventricular contraction (QRS), and ventricular repolarization (QT interval). The electrical signals were in synchrony with optically measured Calcium concentration gradients in terms of d[Ca²⁺]/dt at both whole heart and tissue levels. These microelectrodes therefore provide a real-time analytical tool for monitoring conduction phenotypes of small vertebral animals with a high temporal and spatial resolution.
Authors:
Fei Yu; Yu Zhao; Jie Gu; Katherine L Quigley; Neil C Chi; Yu-Chong Tai; Tzung K Hsiai
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomedical microdevices     Volume:  14     ISSN:  1572-8781     ISO Abbreviation:  Biomed Microdevices     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-03-14     Completed Date:  2012-07-05     Revised Date:  2014-09-21    
Medline Journal Info:
Nlm Unique ID:  100887374     Medline TA:  Biomed Microdevices     Country:  United States    
Other Details:
Languages:  eng     Pagination:  357-66     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Arrhythmias, Cardiac / physiopathology
Calcium / metabolism*
Electrocardiography
Electrophysiologic Techniques, Cardiac*
Electrophysiological Phenomena
Epicardial Mapping / methods*
Heart / physiopathology*
Heart Conduction System
Heart Rate
Heart Ventricles / physiopathology
Ion Transport
Microelectrodes*
Microtechnology / methods
Myocardial Infarction / physiopathology
Regeneration
Signal-To-Noise Ratio
Zebrafish
Grant Support
ID/Acronym/Agency:
HD069305/HD/NICHD NIH HHS; HL083015/HL/NHLBI NIH HHS; HL091302/HL/NHLBI NIH HHS; HL104239/HL/NHLBI NIH HHS; R01 HL083015/HL/NHLBI NIH HHS; R01 HL083015-01A1/HL/NHLBI NIH HHS; R01 HL104239/HL/NHLBI NIH HHS; R21 HL091302/HL/NHLBI NIH HHS; R21 HL091302-01A1/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
SY7Q814VUP/Calcium
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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