| FlbD of Caulobacter crescentus is a homologue of the NtrC (NRI) protein and activates sigma 54-dependent flagellar gene promoters. | |
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MedLine Citation:
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PMID: 2315326 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The periodic transcription of flagellar genes in the Caulobacter crescentus cell cycle is controlled, in part, by their organization in a regulatory hierarchy. The flbG (hook operon), flaN, and flagellin gene operons, which are at the lowest levels of the hierarchy and expressed late in the cell cycle, contain Ntr-like promoters. We report that flbD, one of the early genes required in trans for expression of these operons, codes for a 52-kDa protein homologous to the transcriptional activators NtrC (NRI), NifA, DctD, HydG, and XylR. Our results show that in Escherichia coli flbD partially complements glnG (ntrC) mutations and stimulates transcription of the C. crescentus sigma 54 RNA polymerase-dependent flbG gene. Additionally, the sequence predicts that FlbD protein, along with NtrC, DctD, and HydG proteins, is structurally related at the amino-terminal domain to a larger family of response regulators that mediate cellular responses to environmental stimuli. FlbD may be a singular member of this large protein family in that its function is tied to an internal cell-cycle signal. FlbD is also unusual in that its amino-terminal domain contains only one of the three residues conserved in previously described members of this family of response regulators. |
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Authors:
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G Ramakrishnan; A Newton |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 87 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1990 Mar |
Date Detail:
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Created Date: 1990-04-25 Completed Date: 1990-04-25 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2369-73 Citation Subset: IM |
Affiliation:
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Department of Biology, Lewis Thomas Laboratory, Princeton University, NJ 08544. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/M32065; M69228 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Bacteria / cytology, genetics*, metabolism Base Sequence Cell Cycle DNA, Bacterial / genetics DNA-Directed RNA Polymerases / metabolism Flagella / metabolism* Flagellin / genetics Genes, Bacterial* Molecular Sequence Data Multigene Family Operon Promoter Regions, Genetic* Restriction Mapping Sequence Homology, Nucleic Acid Transcription, Genetic |
| Grant Support | |
ID/Acronym/Agency:
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GM 22299/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 12777-81-0/Flagellin; EC 2.7.7.6/DNA-Directed RNA Polymerases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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