Document Detail


Flavoridin inhibits Yersinia enterocolitica uptake into fibronectin-adherent HeLa cells.
MedLine Citation:
PMID:  15927747     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, three structurally distinct disintegrins (flavoridin, echistatin, kistrin) were used as molecular probes to further characterize the molecular mechanisms underlying Yersinia enterocolitica infection of host cells. The activity of the three disintegrins on Y. enterocolitica uptake into fibronectin-adherent HeLa cells was evaluated at disintegrin doses which were non-cytotoxic and unable to induce cell detachment. Flavoridin resulted to be the most effective in inhibiting bacterial entry into host cells; echistatin was almost 50% less effective than flavoridin, whereas kistrin was definitely inactive. Our results suggest that alpha(5)beta(1) integrin receptor, which binds flavoridin with higher affinity than the other two disintegrins, plays a major role in Y. enterocolitica uptake into HeLa cells. Furthermore, flavoridin binding to this integrin prevented the disruption of the functional complex FAK-Cas, which occurs in the Y. enterocolitica uptake process.
Authors:
Antonio Scibelli; Gianluca Matteoli; Sante Roperto; Elena Alimenti; Ludovico Dipineto; Luigi Michele Pavone; Rossella Della Morte; Lucia Francesca Menna; Alessandro Fioretti; Norma Staiano
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEMS microbiology letters     Volume:  247     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-06-01     Completed Date:  2006-02-06     Revised Date:  2012-06-05    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  51-7     Citation Subset:  IM    
Affiliation:
Dipartimento di Patologia e Sanità Animale, Italy.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Adhesion / drug effects
Crotalid Venoms / pharmacology*
Disintegrins / pharmacology*
Fibronectins
Focal Adhesion Kinase 1 / chemistry,  metabolism
HeLa Cells / drug effects*,  microbiology
Humans
Integrin alpha5beta1 / chemistry,  metabolism
Molecular Probes
Peptides / pharmacology*
Yersinia enterocolitica / drug effects*,  pathogenicity
Chemical
Reg. No./Substance:
0/Crotalid Venoms; 0/Disintegrins; 0/Fibronectins; 0/Integrin alpha5beta1; 0/Molecular Probes; 0/Peptides; 133648-01-8/flavoridin protein, Trimeresurus; EC 2.7.10.1/Focal Adhesion Kinase 1; EC 2.7.10.2/PTK2 protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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