Document Detail

Flavodoxin is required for the activation of the anaerobic ribonucleotide reductase.
MedLine Citation:
PMID:  8267617     Owner:  NLM     Status:  MEDLINE    
The inactive anaerobic ribonucleotide reductase from Escherichia coli is transformed by a multienzyme system and S-adenosylmethionine + NADPH into a radical protein that is enzymatically active. One of the activating enzyme components was earlier shown to be ferredoxin (flavodoxin):NADP+ reductase. Here we present evidence that flavodoxin, but not ferredoxin, also is a component of the system. Light reduced deazaflavin can substitute for the flavodoxin system. An additional unidentified low-molecular weight component further stimulates the reaction.
V Bianchi; R Eliasson; M Fontecave; E Mulliez; D M Hoover; R G Matthews; P Reichard
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  197     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1993 Dec 
Date Detail:
Created Date:  1994-01-25     Completed Date:  1994-01-25     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  792-7     Citation Subset:  IM    
Department of Biochemistry 1, Karolinska Institute, Stockholm, Sweden.
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MeSH Terms
5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / metabolism
Base Sequence
Chromatography, Gel
Cloning, Molecular
DNA Primers
Enzyme Activation
Escherichia coli / enzymology*,  genetics,  growth & development
Flavodoxin / biosynthesis,  isolation & purification,  metabolism*
Genes, Bacterial
Molecular Sequence Data
Polymerase Chain Reaction
Recombinant Proteins / biosynthesis,  isolation & purification,  metabolism
Restriction Mapping
Ribonucleotide Reductases / isolation & purification,  metabolism*
Grant Support
Reg. No./Substance:
0/DNA Primers; 0/Flavodoxin; 0/Recombinant Proteins; EC 1.17.4.-/Ribonucleotide Reductases; EC S-Methyltransferase

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