Document Detail


Flanking Sequence Determination and Event Specific Detection of Transgenic Wheat B72-8-11b Strain.
MedLine Citation:
PMID:  23319185     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Exogenous fragment sequence and flanking sequence between exogenous fragment and recombinant chromosome of transgenic wheat B72-8-11b were successfully acquired through PCR amplification with cross-matched primers from exogenous genes. Newly acquired exogenous fragment covered the full-length sequence of transformed genes such as transformed plasmid and corresponding functional genes including marker uidA, promoter ubiquitin, lacZ, 1Dx5, and part of sequence of the wheat genome. A specific PCR detection method for transgenic wheat B72-8-11b strain was established on the basis of primers designed according to flanking sequence. The designed primers revealed specific amplification of 132 bp product of transgenic wheat B72-8-11b strain. This method is characteristics of high specificity, high reproducibility, rapid identification, and excellent accuracy for the identification of transgenic wheat B72-8-11b strain.
Authors:
Piqiao Zhang; Junyi Xu; Qiuyue Zheng; Fengxia Luan; Jijuan Cao; Hesheng Hou
Related Documents :
14992005 - Molecular identification of six medicinal curcuma plants produced in sichuan: evidence ...
2119815 - Cloning and characterization of the genes for ribosomal proteins l10 and l12 from synec...
2974085 - Structure and organization of marchantia polymorpha chloroplast genome. ii. gene organi...
2460265 - Darkness and antibiotics increase the steady-state transcripts of the elongation factor...
15105995 - Comparative organization of wheat homoeologous group 3s and 7l using wheat-rice synteny...
22383055 - Detection, prevalence and analysis of emerging porcine parvovirus infections.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-16
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  -     ISSN:  1559-0291     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Liaoning Normal University, No.1 Liushu South Street, Gan Jing Zi District, Dalian, 116029, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Gene Cloning and Characterization of a Novel Salt-Tolerant and Glucose-Enhanced ?-Glucosidase from a...
Next Document:  Enhanced Production of Bacterial Cellulose by Using Gluconacetobacter hansenii NCIM 2529 Strain Unde...