Document Detail


Flagellin contamination of recombinant heat shock protein 70 is responsible for its activity on T cells.
MedLine Citation:
PMID:  17178717     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Heat shock proteins (Hsp) 60 and 70 have been intensively studied for their ability to activate innate immunity. Heat shock proteins had been shown to induce the activation of dendritic cells, T cells, and B cells. However, the possible contamination of endotoxin in heat shock protein preparations makes their function as an activator of immune system ambiguous. Here, we examined the ability of bacterial Hsp60 and Hsp70 to activate Jurkat T cells and primary T cells. We found that Burkholderia pseudomallei Hsp70 and Mycobacterium tuberculosis Hsp70 could costimulate Jurkat T cells to make IL-2 and signal through TLR5. This costimulatory activity is not due to endotoxin or contaminants signaling via TLR2 nor TLR4. However, recombinant Hsp70 expressed in Escherichia coli DeltafliC strain completely lost its ability to costimulate T cells. Thus, the activation of T cells by recombinant Hsp70 is ascribed to flagellin contamination.
Authors:
Zhiyong Ye; Yunn-Hwen Gan
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Publication Detail:
Type:  Journal Article     Date:  2006-12-18
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  282     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-02-12     Completed Date:  2007-03-26     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4479-84     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Block MD7, #05-10, 8 Medical Drive, Singapore 117597, Singapore.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / genetics,  immunology*
Burkholderia pseudomallei / genetics,  immunology
Chaperonin 60 / genetics,  immunology
Dendritic Cells / cytology,  immunology*
Escherichia coli / genetics,  immunology
Escherichia coli Proteins / genetics,  immunology
Flagellin / immunology*
HSP70 Heat-Shock Proteins / genetics,  immunology*
Humans
Immunity, Innate
Interleukin-2 / immunology
Jurkat Cells
Lipopolysaccharides / immunology
Lymphocyte Activation / immunology*
Mycobacterium tuberculosis / genetics,  immunology
Recombinant Proteins / genetics,  immunology
Signal Transduction / immunology*
T-Lymphocytes / immunology*
Toll-Like Receptors / immunology
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Chaperonin 60; 0/Escherichia coli Proteins; 0/FliC protein, E coli; 0/HSP70 Heat-Shock Proteins; 0/HSP70 protein, Mycobacterium tuberculosis; 0/IL2 protein, human; 0/Interleukin-2; 0/Lipopolysaccharides; 0/Recombinant Proteins; 0/Toll-Like Receptors; 12777-81-0/Flagellin

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