| Flagellated but not hyperfimbriated Salmonella enterica serovar Typhimurium attaches to and forms biofilms on cholesterol-coated surfaces. | |
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MedLine Citation:
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PMID: 20118264 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The asymptomatic, chronic carrier state of Salmonella enterica serovar Typhi occurs in the bile-rich gallbladder and is frequently associated with the presence of cholesterol gallstones. We have previously demonstrated that salmonellae form biofilms on human gallstones and cholesterol-coated surfaces in vitro and that bile-induced biofilm formation on cholesterol gallstones promotes gallbladder colonization and maintenance of the carrier state. Random transposon mutants of S. enterica serovar Typhimurium were screened for impaired adherence to and biofilm formation on cholesterol-coated Eppendorf tubes but not on glass and plastic surfaces. We identified 49 mutants with this phenotype. The results indicate that genes involved in flagellum biosynthesis and structure primarily mediated attachment to cholesterol. Subsequent analysis suggested that the presence of the flagellar filament enhanced binding and biofilm formation in the presence of bile, while flagellar motility and expression of type 1 fimbriae were unimportant. Purified Salmonella flagellar proteins used in a modified enzyme-linked immunosorbent assay (ELISA) showed that FliC was the critical subunit mediating binding to cholesterol. These studies provide a better understanding of early events during biofilm development, specifically how salmonellae bind to cholesterol, and suggest a target for therapies that may alleviate biofilm formation on cholesterol gallstones and the chronic carrier state. |
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Authors:
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Robert W Crawford; Kristin E Reeve; John S Gunn |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-01-29 |
Journal Detail:
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Title: Journal of bacteriology Volume: 192 ISSN: 1098-5530 ISO Abbreviation: J. Bacteriol. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-27 Completed Date: 2010-06-28 Revised Date: 2011-03-03 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: United States |
Other Details:
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Languages: eng Pagination: 2981-90 Citation Subset: IM |
Affiliation:
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The Ohio State University, Center for Microbial Interface Biology, 460 W. 12th Ave., Biomedical Research Tower, Columbus, OH 43210, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Adhesion
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physiology* Biofilms / growth & development* Cholesterol / chemistry* Fimbriae, Bacterial / genetics, metabolism Flagella / physiology* Gene Expression Regulation, Bacterial Polysaccharides, Bacterial / metabolism Salmonella typhimurium / physiology* Surface Properties |
| Grant Support | |
ID/Acronym/Agency:
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AI066208/AI/NIAID NIH HHS; R01 AI066208-05/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Polysaccharides, Bacterial; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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