| Flagellar membrane agglutination and sexual signaling in the conditional GAM-1 mutant of Chlamydomonas. | |
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MedLine Citation:
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PMID: 701379 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The temperature-sensitive gametogenesis-defective mutant, gam-1 is sex-limited, expressed only in mating type minus (mt-), and can sexually agglutinate but not fuse at the restrictive temperature (35 degrees C) with gametes of wild type (wt) mt+. Thin-section, freeze-cleave, and scanning electron microscopy reveal that the gam-1 phenotype is dependent on both the temperature at which the cells undergo nitrogen starvation (and therefore gamete formation) and the temperature at which the cells are maintained during the 12 h before mating. Under all conditions of gametogenesis at 35 degrees C, each gam-1 cell produces a normal-appearing membrane-associated mating structure that fails to activate in response to flagellar agglutination. Varying with the conditions of gametogenesis, on the other hand, are the agglutination and signaling properties of the gam-1 flagella. The two mutant phenotypes displayed by gam-1 have been denoted gam-1-I and gam-1-II. An agglutination reaction involving gam-1-I cells does not result in activation of the wt mt+ mating structure. A more stable agglutination reaction, which can result in activation of the wt mt+ mating structure, is characteristic of gam-1-II cells, but because the gam-1 mt- mating sturcture still fails to activate, cell fusion is precluded. We conclude that the gam-1 mutation affects flagellar component(s) involved in establishing an effective, signal-generating agglutination reaction. |
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Authors:
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C L Forest; D A Goodenough; U W Goodenough |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: The Journal of cell biology Volume: 79 ISSN: 0021-9525 ISO Abbreviation: J. Cell Biol. Publication Date: 1978 Oct |
Date Detail:
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Created Date: 1978-12-27 Completed Date: 1978-12-27 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 74-84 Citation Subset: IM |
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| MeSH Terms | |
Descriptor/Qualifier:
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Agglutination Chlamydomonas / genetics, physiology*, ultrastructure Crosses, Genetic Flagella / physiology* Genes* Mutation Temperature |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Cell Biol ISSN: 0021-9525 ISSN: 1540-8140 Publisher: The Rockefeller University Press |
Article Information Download PDF  Print publication date: Day: 1 Month: 10 Year: 1978 Volume: 79 Issue: 1 First Page: 74 Last Page: 84 ID: 2110210 Publisher Id: 79027419 PubMed Id: 701379 |
| Flagellar membrane agglutination and sexual signaling in the conditional GAM-1 mutant of Chlamydomonas | |
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