Document Detail


Fla-DGGE analysis of Campylobacter jejuni and Campylobacter coli in cecal samples of broilers without cultivation.
MedLine Citation:
PMID:  17890022     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In a commercial broiler flock during rearing multiple genotypes of Campylobacter jejuni may be present as well as in gastrointestinal tracts of individual birds. The aim of this study was to optimize and apply a denaturing gradient gel electrophoresis assay of the flagellin gene (fla-DGGE) for analysis of C. jejuni and Campylobacter coli in cecal samples of broilers without prior cultivation. One C. coli and 21 C. jejuni strains isolated from broiler flocks, of which 14 typed as unique by restriction fragment length polymorphism of flaA and two undefined strains, were clustered into 9 groups when applying fla-DGGE. Spiking of cecal samples revealed that fla-DGGE is able to detect at least 4.55-5.96logCFUCampylobacter/mlcecal material. The presence of 3 strains spiked in cecal material was demonstrated by fla-DGGE as the corresponding bands were visible on the DGGE gel. Naturally contaminated cecal samples were shown to contain different types of C. jejuni and C. coli. Fla-DGGE has some potential as a cultivation-independent fast primary subtyping method for C. jejuni and C. coli in cecal samples of broilers.
Authors:
Hristo Najdenski; Marc Heyndrickx; Lieve Herman; Winy Messens
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-08-15
Journal Detail:
Title:  Veterinary microbiology     Volume:  127     ISSN:  0378-1135     ISO Abbreviation:  Vet. Microbiol.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2007-12-24     Completed Date:  2008-11-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705469     Medline TA:  Vet Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  196-202     Citation Subset:  IM    
Affiliation:
Institute for Agricultural and Fisheries Research (ILVO), Technology and Food Unit, Brusselsesteenweg 370, Melle, Belgium.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Typing Techniques / methods
Campylobacter Infections / microbiology,  veterinary*
Campylobacter coli / classification*,  genetics*,  isolation & purification
Campylobacter jejuni / classification*,  genetics*,  isolation & purification
Cecum / microbiology*
Chickens
Colony Count, Microbial
Electrophoresis, Polyacrylamide Gel / methods,  veterinary
Flagellin / genetics
Polymerase Chain Reaction / veterinary
Poultry Diseases / microbiology*
Sensitivity and Specificity
Chemical
Reg. No./Substance:
12777-81-0/Flagellin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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