Document Detail


Fixation of vervet monkey oral mucosa for ultrastructural investigation--TEM.
MedLine Citation:
PMID:  3321568     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study was undertaken to determine optimal fixation procedure for vervet monkey (Cercopithecus pygerythrus) oral mucosa. Perfusion and immersion fixation were investigated using glutaraldehyde and glutaraldehyde-paraformaldehyde fixatives with either a phosphate or sodium cacodylate buffer as vehicle and with osmolarities varying from 2010 to 320 mosm. Good fixation could not be obtained uniformly or consistently by perfusion. Vervet monkey oral mucosa is best fixed by first perfusing the head and neck of the animal with 250-500 ml 0.9% saline containing Procaine-HCl and heparin, followed by decapitation and immersion of the head in a 2.5% glutaraldehyde: 2% paraformaldehyde: 0.02 M sodium cacodylate buffered fixative (900 mosm) at 4 C for 24 hr.
Authors:
E S Grossman; J C Austin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Stain technology     Volume:  62     ISSN:  0038-9153     ISO Abbreviation:  Stain Technol     Publication Date:  1987 Sep 
Date Detail:
Created Date:  1988-02-23     Completed Date:  1988-02-23     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0404535     Medline TA:  Stain Technol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  335-9     Citation Subset:  IM    
Affiliation:
Medical Research Council/University of the Witwatersrand Dental Research Institute, Johannesburg, South Africa.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cercopithecus
Gingiva / ultrastructure
Histological Techniques
Microscopy, Electron
Mouth Mucosa / ultrastructure*

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