Document Detail


Fixation and staining of planaria for histological study.
MedLine Citation:
PMID:  7548433     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fixation and staining of planaria can affect the interpretation of histopathological changes following their exposure to various agents. We assessed several fixation protocols with various stains in planaria to determine an optimal combination. Planaria were fixed in each of the following: 10% neutral buffered formalin, 2.5%, glutaraldehyde, Bouin's, Zenker's, 70% ethanol, and relaxant. In addition, planaria were fixed in relaxant and postfixed in each of the fixatives above. Paraffin embedded sections from each fixation protocol were stained with hematoxylin and eosin (H & E), toluidine blue, periodic acid-Schiff (PAS), or phosphotungstic acid-hematoxylin (PTAH). Relaxant fixed planaria were also stained with Steiner's, Holmes, trichrome, Giemsa, Grocott's methenamine silver (GMS) and antibodies for intermediate filaments (cytokeratin, vimentin and desmin). Relaxant and Zenker's gave the best fixation with minimal artifacts. Formalin, glutaraldehyde, and ethanol were unacceptable because they caused contortions of the body, crenation, and a darkly pigmented epidermis. Gastroderm could be differentiated from stroma best when stained with H & E, toluidine blue and PTAH. Other organ systems differentially stained included the epidermis, marginal adhesion gland, nervous tissue, and muscle. PAS, Steiner's, Holmes, trichrome and the intermediate filament stains were not useful for planaria staining. The most morphological information was obtained with relaxant fixative and a combination of sections stained with H & E and PTAH.
Authors:
L L DiCiaula; G L Foley; D J Schaeffer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnic & histochemistry : official publication of the Biological Stain Commission     Volume:  70     ISSN:  1052-0295     ISO Abbreviation:  Biotech Histochem     Publication Date:  1995 May 
Date Detail:
Created Date:  1995-11-06     Completed Date:  1995-11-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9107378     Medline TA:  Biotech Histochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  119-23     Citation Subset:  IM    
Affiliation:
Department of Veterinary Pathobiology, University of Illinois College of Veterinary Medicine, Urbana 61801, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Histological Techniques
Planarians / cytology*
Staining and Labeling / methods
Tissue Fixation / methods

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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