Document Detail


Fixation of neural tissue for electron microscopy with an electronically controlled perfusion pump.
MedLine Citation:
PMID:  3551187     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Many attempts to improve the perfusion of mammalian tissues aim at changes of the osmotic pressure. We describe a method for fixation of nervous tissues controlling both the hydrostatic pressure and the flow rate of a perfusion solution. The constancy of these parameters is guaranteed by an electronically controlled perfusion pump. Thus, a more uniform and complete preservation can be achieved. Further advantages of this method include provision for a rapid succession of rinsing and fixation solution and a continuous control of the hydrostatic pressure during perfusion.
Authors:
P Eichhammer; R Zeller; R Rohkamm
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Tissue & cell     Volume:  19     ISSN:  0040-8166     ISO Abbreviation:  Tissue Cell     Publication Date:  1987  
Date Detail:
Created Date:  1987-05-11     Completed Date:  1987-05-11     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0214745     Medline TA:  Tissue Cell     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  153-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain / ultrastructure*
Histological Techniques
Male
Microscopy, Electron
Perfusion / instrumentation,  methods
Rats
Rats, Inbred Strains

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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