Document Detail

Five polymorphic microsatellite VNTRs on the human X chromosome.
MedLine Citation:
PMID:  2316523     Owner:  NLM     Status:  MEDLINE    
The human genome contains approximately 50,000 copies of an interspersed repeat with the sequence (dT.dG/dA.dC)n, where n = approximately 10-60. We and others have found that several of these repeats have variable lengths in different individuals, with allelic fragments varying in size by multiples of 2 bp. These "microsatellite" variable number of tandem repeats (VNTRs) may be scored by PCR, using unique flanking primers to amplify the repeat-containing regions and resolving the products on DNA sequencing gels. Since few VNTRs have been found on the X chromosome, we screened a flow-sorted X chromosome-specific genomic library for microsatellites. Approximately 25% of the phage clones hybridized to a poly (dT-dG).poly(dA-dC) probe. Of seven X-linked microsatellites present in positive phages, five are polymorphic and three have both eight or more alleles and heterozygosities exceeding 75%. Using PCR to amplify genomic DNAs from hybrid cell panels, we confirmed the X localization of these VNTRs and regionally mapped four of them. The fifth VNTR was regionally mapped by virtue of its tight linkage to DXS87 in Centre du Polymorphisme Humain families. We conclude that whatever factors limit the occurrence of "classical" VNTRs and RFLPs on the X chromosome do not appear to operate in the case of microsatellite VNTRs.
J A Luty; Z Guo; H F Willard; D H Ledbetter; S Ledbetter; M Litt
Related Documents :
9688523 - Specific chromomeres on the chicken w lampbrush chromosome contain specific repetitive ...
9425223 - Expanded cag repeats in swedish spinocerebellar ataxia type 7 (sca7) patients: effect o...
22100493 - Fluorescence in situ hybridization using bacterial artificial chromosome (bac) clones f...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of human genetics     Volume:  46     ISSN:  0002-9297     ISO Abbreviation:  Am. J. Hum. Genet.     Publication Date:  1990 Apr 
Date Detail:
Created Date:  1990-04-23     Completed Date:  1990-04-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370475     Medline TA:  Am J Hum Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  776-83     Citation Subset:  IM    
Department of Biochemistry, Oregon Health Sciences University, Portland 97201-3098.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Chromosome Mapping
DNA, Satellite / genetics*
Genetic Markers
Genome, Human*
Hybrid Cells
Molecular Sequence Data
Polymorphism, Genetic*
Repetitive Sequences, Nucleic Acid*
X Chromosome*
Grant Support
Reg. No./Substance:
0/DNA, Satellite; 0/Genetic Markers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The parental origin of the extra X chromosome in 47,XXX females.
Next Document:  A human alpha satellite DNA subset specific for chromosome 12.