Document Detail


Five polymorphic microsatellite VNTRs on the human X chromosome.
MedLine Citation:
PMID:  2316523     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The human genome contains approximately 50,000 copies of an interspersed repeat with the sequence (dT.dG/dA.dC)n, where n = approximately 10-60. We and others have found that several of these repeats have variable lengths in different individuals, with allelic fragments varying in size by multiples of 2 bp. These "microsatellite" variable number of tandem repeats (VNTRs) may be scored by PCR, using unique flanking primers to amplify the repeat-containing regions and resolving the products on DNA sequencing gels. Since few VNTRs have been found on the X chromosome, we screened a flow-sorted X chromosome-specific genomic library for microsatellites. Approximately 25% of the phage clones hybridized to a poly (dT-dG).poly(dA-dC) probe. Of seven X-linked microsatellites present in positive phages, five are polymorphic and three have both eight or more alleles and heterozygosities exceeding 75%. Using PCR to amplify genomic DNAs from hybrid cell panels, we confirmed the X localization of these VNTRs and regionally mapped four of them. The fifth VNTR was regionally mapped by virtue of its tight linkage to DXS87 in Centre du Polymorphisme Humain families. We conclude that whatever factors limit the occurrence of "classical" VNTRs and RFLPs on the X chromosome do not appear to operate in the case of microsatellite VNTRs.
Authors:
J A Luty; Z Guo; H F Willard; D H Ledbetter; S Ledbetter; M Litt
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of human genetics     Volume:  46     ISSN:  0002-9297     ISO Abbreviation:  Am. J. Hum. Genet.     Publication Date:  1990 Apr 
Date Detail:
Created Date:  1990-04-23     Completed Date:  1990-04-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370475     Medline TA:  Am J Hum Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  776-83     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Oregon Health Sciences University, Portland 97201-3098.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromosome Mapping
DNA, Satellite / genetics*
Female
Genetic Markers
Genome, Human*
Humans
Hybrid Cells
Male
Molecular Sequence Data
Pedigree
Polymorphism, Genetic*
Repetitive Sequences, Nucleic Acid*
X Chromosome*
Grant Support
ID/Acronym/Agency:
HD20619/HD/NICHD NIH HHS; R01-GM32500/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Satellite; 0/Genetic Markers
Comments/Corrections

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