Document Detail


Fission yeast Myo51 is a meiotic spindle pole body component with discrete roles during cell fusion and spore formation.
MedLine Citation:
PMID:  19887589     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Class V myosins are dimeric actin-associated motor proteins that deliver cellular cargoes to discrete cellular locations. Fission yeast possess two class V myosins, Myo51 and Myo52. Although Myo52 has been shown to have roles in vacuole distribution, cytokinesis and cell growth, Myo51 has no as yet discernible function in the vegetative life cycle. Here, we uncover distinct functions for this motor protein during mating and meiosis. Not only does Myo51 transiently localise to a foci at the site of cell fusion upon conjugation, but overexpression of the Myo51 globular tail also leads to disruption of cell fusion. Upon completion of meiotic prophase Myo51 localises to the outside of the spindle pole bodies (SPBs), where it remains until completion of meiosis II. Association of Myo51 with SPBs is not dependent upon actin or the septation initiation network (SIN); however, it is dependent on a stable microtubule cytoskeleton and the presence of the Cdc2-CyclinB complex. We observe a rapid and dynamic exchange of Myo51 at the SPB during meiosis I but not meiosis II. Finally, we show that Myo51 has an important role in regulating spore formation upon completion of meiosis.
Authors:
Alex Doyle; Rebeca Martín-García; Arthur T Coulton; Steve Bagley; Daniel P Mulvihill
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-03
Journal Detail:
Title:  Journal of cell science     Volume:  122     ISSN:  1477-9137     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-11-20     Completed Date:  2010-02-01     Revised Date:  2014-10-09    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  4330-40     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Cycle / genetics,  physiology*
Meiosis / genetics,  physiology
Microscopy, Fluorescence
Myosin Type V / genetics,  metabolism,  physiology*
Schizosaccharomyces / metabolism*
Schizosaccharomyces pombe Proteins / genetics,  metabolism,  physiology*
Spindle Apparatus / metabolism*
Spores, Fungal / growth & development*,  metabolism
Grant Support
ID/Acronym/Agency:
BB/F011784/1//Biotechnology and Biological Sciences Research Council; JF20605//Biotechnology and Biological Sciences Research Council; //Biotechnology and Biological Sciences Research Council; //Cancer Research UK
Chemical
Reg. No./Substance:
0/Schizosaccharomyces pombe Proteins; EC 3.6.1.-/Myosin Type V

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